Detailed Information on Publication Record
2001
Determination of esmolol in serum by capillary zone electrophoresis and its monitoring in course of heart surgery
MALOVANÁ, Sabina, Dagmar GAJDOŠOVÁ, Jaroslav BENEDÍK and Josef HAVELBasic information
Original name
Determination of esmolol in serum by capillary zone electrophoresis and its monitoring in course of heart surgery
Name (in English)
Determination of esmolol in serum by capillary zone electrophoresis and its monitoring in course of heart surgery
Authors
MALOVANÁ, Sabina, Dagmar GAJDOŠOVÁ, Jaroslav BENEDÍK and Josef HAVEL
Edition
Journal of Chromatography B, 2001
Other information
Type of outcome
Článek v odborném periodiku
Confidentiality degree
není předmětem státního či obchodního tajemství
Organization unit
Faculty of Science
UT WoS
000170358700004
Keywords in English
beta-blockers; Esmolol
Tags
Změněno: 21/11/2001 14:50, Ing. Dagmar Gajdošová
V originále
A new Capillary Zone Electrophoresis (CZE) procedure for determination of esmolol, an ultra-short-acting -blocker, in serum was developed. Dichloromethane was applied as a deproteination agent and it was used also for the inactivation of erythrocytal esterase and in the same time for the extraction of esmolol from blood. The re-extraction of esmolol from organic phase to water phase was performed by 0.01 M HCl. An aliquot of 200 ml of acid aqueous phase was used for the injection and analysis. CZE determination was done in 50 mM phosphate buffer (pH=8.0) with detection at 222 nm. The concentration detection limit of esmolol in serum was 0.051 g/ml. This method was applied in an extensive heart surgery experiment on pigs (Sus scrofa).
In English
A new Capillary Zone Electrophoresis (CZE) procedure for determination of esmolol, an ultra-short-acting -blocker, in serum was developed. Dichloromethane was applied as a deproteination agent and it was used also for the inactivation of erythrocytal esterase and in the same time for the extraction of esmolol from blood. The re-extraction of esmolol from organic phase to water phase was performed by 0.01 M HCl. An aliquot of 200 ml of acid aqueous phase was used for the injection and analysis. CZE determination was done in 50 mM phosphate buffer (pH=8.0) with detection at 222 nm. The concentration detection limit of esmolol in serum was 0.051 g/ml. This method was applied in an extensive heart surgery experiment on pigs (Sus scrofa).
Links
| MSM 143100011, plan (intention) |
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