2001
Partial characterisation of a colored metabolite of Acidithiobacillus ferrooxidans grown on elemental sulfur
ČEŠKOVÁ, Pavla, Zdirad ŽÁK, Jan HAVLIŠ, Barrie JOHNSON, Oldřich JANICZEK et. al.Základní údaje
Originální název
Partial characterisation of a colored metabolite of Acidithiobacillus ferrooxidans grown on elemental sulfur
Autoři
ČEŠKOVÁ, Pavla, Zdirad ŽÁK, Jan HAVLIŠ, Barrie JOHNSON, Oldřich JANICZEK a Martin MANDL
Vydání
Amsterdam, Biohydrometallurgy: Fundamentals, Technology and Sustainable Development, s. 363-367, 2001
Nakladatel
Elsevier
Další údaje
Jazyk
angličtina
Typ výsledku
Kapitola resp. kapitoly v odborné knize
Obor
10600 1.6 Biological sciences
Stát vydavatele
Nizozemské království
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/01:00004415
Organizační jednotka
Přírodovědecká fakulta
ISBN
0 444 50623 3
Klíčová slova anglicky
Thiobacillus; Acidithiobacillus; sulfur oxidation; X-ray structure analysis
Změněno: 30. 10. 2001 10:11, doc. Ing. Martin Mandl, CSc.
Anotace
V originále
The aim of the study was the physico-chemical characterisation of a violet-colored metabolite isolated from a culture of Acidithiobacillus ferrooxidans grown on elemental sulfur. The metabolite, which was bound to the surface of the bacterial cells, was detectable visually after prolonged culture incubation, corresponding to a pH change from about pH 4 to about pH 0.5. When extracted from the bacteria, the metabolite was found to be hydrophobic and insoluble in water. X-ray structure analysis, mass spectroscopy, and NMR were used to elucidate the chemical structure of this metabolite. The results of all these methods were consistent, and confirmed the existence of aromatic pyrene rings which were connected, probably via aliphatic chains, to cell exopolymers. The amount of the metabolite produced varied from culture to culture, though the conditions which promote its production are currently unknown. The proposed chemical structure of the colored metabolite indicated a possible role in the adsorption of At. ferrooxidans cells onto particulate elemental sulfur.
Návaznosti
GA525/00/0785, projekt VaV |
| ||
MSM 143100005, záměr |
|