J 2001

Determination of Metallothionein at Femtomole Level by Constant Current Stripping Chronopotentiometry

KIZEK, René, Libuše TRNKOVÁ and Emil PALEČEK

Basic information

Original name

Determination of Metallothionein at Femtomole Level by Constant Current Stripping Chronopotentiometry

Authors

KIZEK, René, Libuše TRNKOVÁ and Emil PALEČEK

Edition

Anal. Chem. USA, American Chemical Society, 2001, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.532

RIV identification code

RIV/00216224:14310/01:00004521

Organization unit

Faculty of Science

Keywords in English

Metallothionein; Constant current stripping chronopotentiometry; Catalytic hydrogen evolution; Cyprinus carpio
Změněno: 8/11/2001 15:15, prof. RNDr. Libuše Trnková, CSc.

Abstract

V originále

Metallothionein (MT) from rabbit liver was analysed by differential pulse polarography (DPP with DME), cyclic voltammetry (CV), square wave voltammetry (SWV), and chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode (HMDE) under various conditions. The highest sensitivity of the MT determination was obtained with CPSA which produced a well developed peak H due to catalytic hydrogen evolution at highly negative potentials. The highest peak H was obtained in borate buffer at pH 7.6. In this medium subnanomolar concentrations of MT were detectable. In the adsorptive transfer stripping (medium exchange) experiments determination of few femtomoles of MT in 5 ml aliquots of the analyte was possible. CPSA determination of MT in various tissues of carp (Cyprinus cyprio) yielded values in agreement with the published data.

Links

GV204/97/K084, research and development project
Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu
Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research
MSM 143100011, plan (intention)
Name: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů
Investor: Ministry of Education, Youth and Sports of the CR, Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles