Detailed Information on Publication Record
2001
Determination of Metallothionein at Femtomole Level by Constant Current Stripping Chronopotentiometry
KIZEK, René, Libuše TRNKOVÁ and Emil PALEČEKBasic information
Original name
Determination of Metallothionein at Femtomole Level by Constant Current Stripping Chronopotentiometry
Authors
KIZEK, René, Libuše TRNKOVÁ and Emil PALEČEK
Edition
Anal. Chem. USA, American Chemical Society, 2001, 0003-2700
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10405 Electrochemistry
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 4.532
RIV identification code
RIV/00216224:14310/01:00004521
Organization unit
Faculty of Science
Keywords in English
Metallothionein; Constant current stripping chronopotentiometry; Catalytic hydrogen evolution; Cyprinus carpio
Tags
Změněno: 8/11/2001 15:15, prof. RNDr. Libuše Trnková, CSc.
Abstract
V originále
Metallothionein (MT) from rabbit liver was analysed by differential pulse polarography (DPP with DME), cyclic voltammetry (CV), square wave voltammetry (SWV), and chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode (HMDE) under various conditions. The highest sensitivity of the MT determination was obtained with CPSA which produced a well developed peak H due to catalytic hydrogen evolution at highly negative potentials. The highest peak H was obtained in borate buffer at pH 7.6. In this medium subnanomolar concentrations of MT were detectable. In the adsorptive transfer stripping (medium exchange) experiments determination of few femtomoles of MT in 5 ml aliquots of the analyte was possible. CPSA determination of MT in various tissues of carp (Cyprinus cyprio) yielded values in agreement with the published data.
Links
GV204/97/K084, research and development project |
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MSM 143100011, plan (intention) |
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