BRZOBOHATÝ, Břetislav, Todor Nikolaev GENKOV, Ian MOORE, Jaroslava DUBOVÁ, Eliška NEJEDLÁ, Jiří MALBECK and Martin VÁGNER. Developmental Consequences of ipt Activation in Germinating Tobacco Seedlings. In 17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts. Brno: Mendel University of Agriculture and Forestry, Brno, 2001, p. 57. ISBN 80-7157-518-6.
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Basic information
Original name Developmental Consequences of ipt Activation in Germinating Tobacco Seedlings
Authors BRZOBOHATÝ, Břetislav (203 Czech Republic), Todor Nikolaev GENKOV, Ian MOORE, Jaroslava DUBOVÁ (203 Czech Republic), Eliška NEJEDLÁ (203 Czech Republic), Jiří MALBECK and Martin VÁGNER.
Edition Brno, 17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts, p. 57-57, 2001.
Publisher Mendel University of Agriculture and Forestry, Brno
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/01:00004563
Organization unit Faculty of Science
ISBN 80-7157-518-6
Keywords (in Czech) transkripčně aktivační systém;izopentenyltransferáza; cytokininy; tabák; Arabidopsis
Keywords in English transcription activation system; isopentenyl transferase; cytokinins; tobacco; Arabidopsis
Changed by Changed by: RNDr. Eliška Nejedlá, CSc., učo 622. Changed: 26/3/2010 13:10.
Abstract
A transcription activation system based on a synthetic promoter pOp and a corresponding chimeric transcription activator LhG4 was employed to achieve regulatable expression of isopentenyl transferase, IPT, in transgenic tobacco. The system allowed us to analyze in detail developmental consequences of ipt activation in germination and early seedling development. Here we present analysis of (i) dynamics of changes in cytokinin metabolism upon ipt activation and (ii) subsequent chain of developmental alterations leading to transition from originally almost normal seedling to a completely distinct teratoma structure. Major differences in tobacco and Arabidopsis response to ipt activation during germination will be discussed.
Links
IAA5004001, research and development projectName: Systém aktivace transkripce pro studium vztahu mezi metabolizmem a účinkem cytokininů u Arabidopsis a tabáku
Investor: Academy of Sciences of the Czech Republic, Transkription activation system for studying the relationship between cytokinin metabolism and action in Arabidopsis and tobacco
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