| LEXA, Matej, Todor Nikolaev GENKOV a Břetislav BRZOBOHATÝ. Nitrate Reductase Activity in ipt-Expressing Tobacco. In 17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts. Brno: Mendel University of Agriculture and Forestry, Brno. s. 189. ISBN 80-7157-518-6. 2001. |
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@inproceedings{372753,
author = {Lexa, Matej and Genkov, Todor Nikolaev and Brzobohatý, Břetislav},
address = {Brno},
booktitle = {17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts},
language = {eng},
location = {Brno},
isbn = {80-7157-518-6},
pages = {189-189},
publisher = {Mendel University of Agriculture and Forestry, Brno},
title = {Nitrate Reductase Activity in ipt-Expressing Tobacco.},
year = {2001}
}
TY - JOUR
ID - 372753
AU - Lexa, Matej - Genkov, Todor Nikolaev - Brzobohatý, Břetislav
PY - 2001
TI - Nitrate Reductase Activity in ipt-Expressing Tobacco.
PB - Mendel University of Agriculture and Forestry, Brno
CY - Brno
SN - 8071575186
N2 - Using a novel system for expressing ipt gene in tobacco, we were able to increase cytokinins in plant tissue. This enabled us to study new regulatory aspects of nitrate (NO3-) reduction. We have grown plants with elevated cytokinins on MS with variable NO3- and benzyladenine (BA). We determined nitrate reductase activity (NRA) in this plant material. Initially, we found that ipt-expressing plants always display lower NRA than wild-type SR1 plants. We also found that long-term exposure of tobacco to cytokinins caused up to 60% decrease in NRA. However, addition of BA to N-starved plants caused induction of NRA to levels up to 100% higher than those before induction. In ipt-expressing plants this effect was only present if BA was administered together with NO3-. Exposure to 40mM NO3- caused up to 550% induction of NRA. This effect was always more pronounced in wild-type plants. We propose that regulation of NR by NO3- and cytokinins is a result of overlaying regulatory processes, with short-term ones having positive effect on NRA and those acting over extended periods having inhibitory effects on NRA.
ER -
LEXA, Matej, Todor Nikolaev GENKOV a Břetislav BRZOBOHATÝ. Nitrate Reductase Activity in ipt-Expressing Tobacco. In \textit{17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts}. Brno: Mendel University of Agriculture and Forestry, Brno. s.~189. ISBN~80-7157-518-6. 2001.
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