Lack of association between atopic asthma and the tumor
necrosis factor alpha -308 gene polymorphism in a Czech
population

J 2002

Lack of association between atopic asthma and the tumor necrosis factor alpha -308 gene polymorphism in a Czech population

BUČKOVÁ, Dana, Lydie IZAKOVIČOVÁ HOLLÁ, Anna VAŠKŮ, Vladimír ZNOJIL, Jiří VÁCHA et. al.

Basic information

Original name

Lack of association between atopic asthma and the tumor necrosis factor alpha -308 gene polymorphism in a Czech population

Authors

BUČKOVÁ, Dana (203 Czech Republic, guarantor), Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic), Anna VAŠKŮ (203 Czech Republic), Vladimír ZNOJIL (203 Czech Republic) and Jiří VÁCHA (203 Czech Republic)

Edition

Journal of Investigational Allergology and Clinical Immunology, Spain, INTERASMA, 2002, 1018-9068

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Spain

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.604

RIV identification code

RIV/00216224:14110/02:00004573

Organization unit

Faculty of Medicine

UT WoS

000181772300006

Keywords in English

TNF; gene; polymorphism; lymphotoxin; asthma; allergy

Abstract

V originále

Susceptibility to the development of asthma and other atopic diseases is known to be associated with genetic components. Several investigators have linked the tumor necrosis factor (TNF) genes and nearby markers located on chromosome 6p to atopy and asthma. Recent study has demonstrated that the TNF-alpha*2 allele of a polymorphism in the TNF-alpha; gene promoter region (G-308 A) is associated with a higher risk for the development of atopy in Spanish patients. The objective of this study was to evaluate the possible role of two described bi-allelic polymorphisms in the TNF locus alpha;a G to A transition at position -308 in the promoter region of the TNF-alpha gene and an NcoI restriction fragment length polymorphism (RFLP) in the first intron (+252A/G) of the LT-alpha(TNF-beta) gene in atopic diseases in a Czech population. We investigated the distribution of these polymorphisms in a case-control study. The genotypes were determined in 151 patients with atopic asthma and 155 random-sampled control subjects. The genotype frequencies for both polymorphisms were similar in cases and controls. No significant differences in allele frequencies were found between either of the patients groups and the reference subjects. Similarly, there were no associations of any of the examined variants of the TNF genes with total IgE, specific IgE or pulmonary function tests in patients with allergic diseases. We conclude that these polymorphisms of the TNF genes are unlikely to contribute to atopic disease risk in our population. Significant associations which have been reported in other studies may express genetic heterogeneity of these complex diseases.

Links

MSM 141100002, plan (intention)

Name: Molekulární patofyziologie multigenních chorob

Investor: Ministry of Education, Youth and Sports of the CR, Molecular pathophysiology of multigene diseases

Citovat

BUČKOVÁ, Dana, Lydie IZAKOVIČOVÁ HOLLÁ, Anna VAŠKŮ, Vladimír ZNOJIL and Jiří VÁCHA. Lack of association between atopic asthma and the tumor necrosis factor alpha -308 gene polymorphism in a Czech population. Journal of Investigational Allergology and Clinical Immunology. Spain: INTERASMA, 2002, vol. 12, No 3, p. 192-197. ISSN 1018-9068.

@article{372773,
   author = {Bučková, Dana and Izakovičová Hollá, Lydie and Vašků, Anna and Znojil, Vladimír and Vácha, Jiří},
   article_location = {Spain},
   article_number = {3},
   keywords = {TNF; gene; polymorphism; lymphotoxin; asthma; allergy},
   language = {eng},
   issn = {1018-9068},
   journal = {Journal of Investigational Allergology and Clinical Immunology},
   title = {Lack of association between atopic asthma and the tumor necrosis factor alpha -308 gene polymorphism in a Czech population},
   volume = {12},
   year = {2002}
}

TY  - JOUR
ID  - 372773
AU  - Bučková, Dana - Izakovičová Hollá, Lydie - Vašků, Anna - Znojil, Vladimír - Vácha, Jiří
PY  - 2002
TI  - Lack of association between atopic asthma and the tumor necrosis factor alpha -308 gene polymorphism in a Czech population
JF  - Journal of Investigational Allergology and Clinical Immunology
VL  - 12
IS  - 3
SP  - 192
EP  - 192
PB  - INTERASMA
SN  - 10189068
KW  - TNF
KW  - gene
KW  - polymorphism
KW  - lymphotoxin
KW  - asthma
KW  - allergy
N2  - Susceptibility to the development of asthma and other atopic diseases is known to be associated with genetic components. Several investigators have linked the tumor necrosis factor (TNF) genes and nearby markers located on chromosome 6p to atopy and asthma. Recent study has demonstrated that the TNF-alpha*2 allele of a polymorphism in the TNF-alpha; gene promoter region (G-308 A) is associated with a higher risk for the development of atopy in Spanish patients. The objective of this study was to evaluate the possible role of two described bi-allelic polymorphisms in the TNF locus alpha;a G to A transition at position -308 in the promoter region of the TNF-alpha gene and an NcoI restriction fragment length polymorphism (RFLP) in the first intron (+252A/G) of the LT-alpha(TNF-beta) gene in atopic diseases in a Czech population. We investigated the distribution of these polymorphisms in a case-control study. The genotypes were determined in 151 patients with atopic asthma and 155 random-sampled control subjects. The genotype frequencies for both polymorphisms were similar in cases and controls. No significant differences in allele frequencies were found between either of the patients groups and the reference subjects. Similarly, there were no associations of any of the examined variants of the TNF genes with total IgE, specific IgE or pulmonary function tests in patients with allergic diseases. We conclude that these polymorphisms of the TNF genes are unlikely to contribute to atopic disease risk in our population. Significant associations which have been reported in other studies may express genetic heterogeneity of these complex diseases.
ER  -

BUČKOVÁ, Dana, Lydie IZAKOVIČOVÁ HOLLÁ, Anna VAŠKŮ, Vladimír ZNOJIL and Jiří VÁCHA. Lack of association between atopic asthma and the tumor necrosis factor alpha -308 gene polymorphism in a Czech population. \textit{Journal of Investigational Allergology and Clinical Immunology}. Spain: INTERASMA, 2002, vol.~12, No~3, p.~192-197. ISSN~1018-9068.

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