D 2001

Electrochemical investigation of rabbit liver metallothionein at a mercury electrode

TRNKOVÁ, Libuše, René KIZEK and Emil PALEČEK

Basic information

Original name

Electrochemical investigation of rabbit liver metallothionein at a mercury electrode

Authors

TRNKOVÁ, Libuše, René KIZEK and Emil PALEČEK

Edition

Bratislava, Slovakia, XVIth International Symposium on Bioelectrochemistry and Bioenergetics, p. 25-25, BEB, 2001

Publisher

Comenius University, Bratislava, Slovakia

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10405 Electrochemistry

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/01:00004610

Organization unit

Faculty of Science

Keywords in English

rabbit liver metallothionein;constant current chronopotentiometric stripping analysis (CPSA); catalytic hydrogen evolution
Změněno: 9/11/2001 18:45, prof. RNDr. Libuše Trnková, CSc.

Abstract

V originále

In this contribution we have studied a rabbit liver metallothionein by constant current chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode. The highest sensitivity of the MT determination was obtained with CPSA. In (dE/dt)-1 - E coordinates rabbit liver metallothionein gives the well developed peak H. This H peak, produced due to catalytic hydrogen evolution at highly negative potentials, is sharp and reproducible [4]. The shape, height, and position of the H peak were studied under varying experimental conditions - pH, supporting electrolyte composition, time of accumulation, temperature, concentrations of MT and buffers, and the presence or absence of oxygen and metals in solutions.

Links

GV204/97/K084, research and development project
Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu
Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research
MSM 143100011, plan (intention)
Name: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů
Investor: Ministry of Education, Youth and Sports of the CR, Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles