D 2001

Electrochemical investigation of rabbit liver metallothionein at a mercury electrode

TRNKOVÁ, Libuše, René KIZEK and Emil PALEČEK

Basic information

Original name

Electrochemical investigation of rabbit liver metallothionein at a mercury electrode

Authors

TRNKOVÁ, Libuše, René KIZEK and Emil PALEČEK

Edition

Bratislava, Slovakia, XVIth International Symposium on Bioelectrochemistry and Bioenergetics, p. 25-25, BEB, 2001

Publisher

Comenius University, Bratislava, Slovakia

Other information

Language

English

Type of outcome

Proceedings paper

Field of Study

10405 Electrochemistry

Country of publisher

Slovakia

Confidentiality degree

is not subject to a state or trade secret

RIV identification code

RIV/00216224:14310/01:00004610

Organization unit

Faculty of Science

Keywords in English

rabbit liver metallothionein;constant current chronopotentiometric stripping analysis (CPSA); catalytic hydrogen evolution
Changed: 9/11/2001 18:45, prof. RNDr. Libuše Trnková, CSc.

Abstract

V originále

In this contribution we have studied a rabbit liver metallothionein by constant current chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode. The highest sensitivity of the MT determination was obtained with CPSA. In (dE/dt)-1 - E coordinates rabbit liver metallothionein gives the well developed peak H. This H peak, produced due to catalytic hydrogen evolution at highly negative potentials, is sharp and reproducible [4]. The shape, height, and position of the H peak were studied under varying experimental conditions - pH, supporting electrolyte composition, time of accumulation, temperature, concentrations of MT and buffers, and the presence or absence of oxygen and metals in solutions.

Links

GV204/97/K084, research and development project
Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu
Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research
MSM 143100011, plan (intention)
Name: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů
Investor: Ministry of Education, Youth and Sports of the CR, Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles