J 2001

Measurement of small scalar and resiudal dipolar couplings in purine and pyrimidine bases

ŽÍDEK, Lukáš, Haihong WU, Juli FEIGON and Vladimír SKLENÁŘ

Basic information

Original name

Measurement of small scalar and resiudal dipolar couplings in purine and pyrimidine bases

Authors

ŽÍDEK, Lukáš (203 Czech Republic), Haihong WU, Juli FEIGON and Vladimír SKLENÁŘ (203 Czech Republic, guarantor)

Edition

Journal of Biomolecular NMR, Dordrecht, Kluwer/Escom, 2001, 0925-2738

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10403 Physical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.636

RIV identification code

RIV/00216224:14310/01:00004651

Organization unit

Faculty of Science

Keywords in English

NMR methods; nucleic acid bases; residual dipolar couplings; spin-state-selective filter
Změněno: 20/6/2008 12:40, prof. RNDr. Vladimír Sklenář, DrSc.

Abstract

V originále

A suite of spin-state-selective excitation (S3E) NMR experiments for the measurements of small one-bond (13C - 13C, 13C - 15N) and two-bond (1H - 13C, 1H - 15N) coupling constants in 13C, 15N labeled purine and pyrimidine bases is presented. The incorporation of band-selective shaped pulses, elimination of the cross talk between á and â sub-spectra, and accuracy and precision of the proposed approach are discussed. Merits of using S3 E rather than á/â-half-filter are demonstrated using results obtained on isotopicaly labeled DNA oligonucleotides.

Links

LN00A016, research and development project
Name: BIOMOLEKULÁRNÍ CENTRUM
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Center