ŽÍDEK, Lukáš, Haihong WU, Juli FEIGON and Vladimír SKLENÁŘ. Measurement of small scalar and resiudal dipolar couplings in purine and pyrimidine bases. Journal of Biomolecular NMR. Dordrecht: Kluwer/Escom, 2001, vol. 21, No 2, p. 153-159. ISSN 0925-2738.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Measurement of small scalar and resiudal dipolar couplings in purine and pyrimidine bases
Authors ŽÍDEK, Lukáš (203 Czech Republic), Haihong WU, Juli FEIGON and Vladimír SKLENÁŘ (203 Czech Republic, guarantor).
Edition Journal of Biomolecular NMR, Dordrecht, Kluwer/Escom, 2001, 0925-2738.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10403 Physical chemistry
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 4.636
RIV identification code RIV/00216224:14310/01:00004651
Organization unit Faculty of Science
Keywords in English NMR methods; nucleic acid bases; residual dipolar couplings; spin-state-selective filter
Tags NMR methods, nucleic acid bases, residual dipolar couplings, spin-state-selective filter
Changed by Changed by: prof. RNDr. Vladimír Sklenář, DrSc., učo 2611. Changed: 20/6/2008 12:40.
Abstract
A suite of spin-state-selective excitation (S3E) NMR experiments for the measurements of small one-bond (13C - 13C, 13C - 15N) and two-bond (1H - 13C, 1H - 15N) coupling constants in 13C, 15N labeled purine and pyrimidine bases is presented. The incorporation of band-selective shaped pulses, elimination of the cross talk between á and â sub-spectra, and accuracy and precision of the proposed approach are discussed. Merits of using S3 E rather than á/â-half-filter are demonstrated using results obtained on isotopicaly labeled DNA oligonucleotides.
Links
LN00A016, research and development projectName: BIOMOLEKULÁRNÍ CENTRUM
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Center
PrintDisplayed: 26/4/2024 10:14