Identification of Staphylococcus aureus based on PCR
amplification of species specific genomic 826 bp sequence
derived from a common 44-kb SmaI restriction fragment

J 2001

Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb SmaI restriction fragment

ŠTĚPÁN, Jiří, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ, Stanislav ROSYPAL, Václav HÁJEK et. al.

Basic information

Original name

Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb SmaI restriction fragment

Authors

ŠTĚPÁN, Jiří (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic), Vladislava RŮŽIČKOVÁ (203 Czech Republic), Stanislav ROSYPAL (203 Czech Republic), Václav HÁJEK and Jiří DOŠKAŘ (203 Czech Republic, guarantor)

Edition

Molecular and Cellular Probes, London, Academic Press LTD. 2001, 0890-8508

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

[Abstract] [Full text]

Impact factor

Impact factor: 1.588

RIV identification code

RIV/00216224:14310/01:00004693

Organization unit

Faculty of Science

Keywords in English

Staphylococcus aureus; polymerase chain reaction; species-specific probe; 44-kb SmaI fragment L of NCTC 8325; molecular identification

Abstract

V originále

Primers were designed for polymerase chain reaction (PCR)-amplification of a genomic sequence specific to Staphylococcus aureus strains. The sequence corresponds to a part of the 44-kb Smal fragment (fragment L on the S. aureus NCTC 8325 restriction map) which was found to be common to strains of the S. aureus species (Pantucek et al., 1996, International journal of Systematic Bacteriology, 46: 216-222). The labelled 44-kb Smal restriction fragment derived from S. aureus NCTC 8325-4 was hybridized to the EcoRI restriction patterns of genomic DNA from 13 strains representing different macrorestriction types of S. aureus subsp. aureus. This made it possible to reveal the 2052 bp EcoRI restriction subfragment and to demonstrate its presence in all the tested strains. From the sequence of this subfragment, primers were designed by means of which the 826 bp amplicons were obtained in all 216 tested strains of S. aureus. No hybridization and PCR-products were observed in 40 collection strains of other staphylococcal species and subspecies as well as in 45 clinical strains of coagulase-negative staphylococci. These results lead us to the conclusion that the use of the above primers makes it possible to identify rapidly and reliably S. aureus strains of various provenance and different genotypes. (C) 2001 Academic Press.

Links

GA301/99/D075, research and development project

Name: Genotypová diagnostika a typizace klinicky významných koaguláza negativních stafylokoků

Investor: Czech Science Foundation, Genome-based diagnostics and typing of coagulase-negative staphylococci from human clinical specimens

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

ŠTĚPÁN, Jiří, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ, Stanislav ROSYPAL, Václav HÁJEK and Jiří DOŠKAŘ. Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb SmaI restriction fragment. Molecular and Cellular Probes. London: Academic Press LTD., 2001, vol. 15, No 5, p. 249-257. ISSN 0890-8508.

@article{377152,
   author = {Štěpán, Jiří and Pantůček, Roman and Růžičková, Vladislava and Rosypal, Stanislav and Hájek, Václav and Doškař, Jiří},
   article_location = {London},
   article_number = {5},
   keywords = {Staphylococcus aureus; polymerase chain reaction; species-specific probe; 44-kb SmaI fragment L of NCTC 8325; molecular identification},
   language = {eng},
   issn = {0890-8508},
   journal = {Molecular and Cellular Probes},
   title = {Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb SmaI restriction fragment},
   url = {http://www.sciencedirect.com/science?_ob=GatewayURL&_method=citationSearch&_uoikey=B6WNC-45V28XV-2&_origin=EMFR&_version=1&md5=a2a1f6b4ad4d84c00a133bbf867e31cd},
   volume = {15},
   year = {2001}
}

TY  - JOUR
ID  - 377152
AU  - Štěpán, Jiří - Pantůček, Roman - Růžičková, Vladislava - Rosypal, Stanislav - Hájek, Václav - Doškař, Jiří
PY  - 2001
TI  - Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb SmaI restriction fragment
JF  - Molecular and Cellular Probes
VL  - 15
IS  - 5
SP  - 249
EP  - 249
PB  - Academic Press LTD.
SN  - 08908508
KW  - Staphylococcus aureus
KW  - polymerase chain reaction
KW  - species-specific probe
KW  - 44-kb SmaI fragment L of NCTC 8325
KW  - molecular identification
UR  - http://www.sciencedirect.com/science?_ob=GatewayURL&_method=citationSearch&_uoikey=B6WNC-45V28XV-2&_origin=EMFR&_version=1&md5=a2a1f6b4ad4d84c00a133bbf867e31cd
N2  - Primers were designed for polymerase chain reaction (PCR)-amplification of a genomic sequence specific to Staphylococcus aureus strains. The sequence corresponds to a part of the 44-kb Smal fragment (fragment L on the S. aureus NCTC 8325 restriction map) which was found to be common to strains of the S. aureus species (Pantucek et al., 1996, International journal of Systematic Bacteriology, 46: 216-222). The labelled 44-kb Smal restriction fragment derived from S. aureus NCTC 8325-4 was hybridized to the EcoRI restriction patterns of genomic DNA from 13 strains representing different macrorestriction types of S. aureus subsp. aureus. This made it possible to reveal the 2052 bp EcoRI restriction subfragment and to demonstrate its presence in all the tested strains. From the sequence of this subfragment, primers were designed by means of which the 826 bp amplicons were obtained in all 216 tested strains of S. aureus. No hybridization and PCR-products were observed in 40 collection strains of other staphylococcal species and subspecies as well as in 45 clinical strains of coagulase-negative staphylococci. These results lead us to the conclusion that the use of the above primers makes it possible to identify rapidly and reliably S. aureus strains of various provenance and different genotypes. (C) 2001 Academic Press.
ER  -

ŠTĚPÁN, Jiří, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ, Stanislav ROSYPAL, Václav HÁJEK and Jiří DOŠKAŘ. Identification of $<$I$>$Staphylococcus aureus$<$/I$>$ based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb $<$I$>$Sma$<$/I$>$I restriction fragment. \textit{Molecular and Cellular Probes}. London: Academic Press LTD., 2001, vol.~15, No~5, p.~249-257. ISSN~0890-8508.

Detailed Information on Publication Record