| SÝKOROVÁ, Eva, Jiří FAJKUS, Ito MIKAKO and Kiichi FUKUI. Transition between two forms of heterochromatin at plant subtelomeres. Chromosome Research. Dordrecht: Kluwer Academic Publishers, 2001, roč. 9, January, p. 309-323. ISSN 0967-3849. |
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@article{377892,
author = {Sýkorová, Eva and Fajkus, Jiří and Mikako, Ito and Fukui, Kiichi},
article_location = {Dordrecht},
article_number = {January},
keywords = {chromatin structure; nucleosome positioning; plant telomeres and subtelomeres},
language = {eng},
issn = {0967-3849},
journal = {Chromosome Research},
title = {Transition between two forms of heterochromatin at plant subtelomeres},
volume = {roč. 9},
year = {2001}
}
TY - JOUR
ID - 377892
AU - Sýkorová, Eva - Fajkus, Jiří - Mikako, Ito - Fukui, Kiichi
PY - 2001
TI - Transition between two forms of heterochromatin at plant subtelomeres
JF - Chromosome Research
VL - roč. 9
IS - January
SP - 309
EP - 309
PB - Kluwer Academic Publishers
SN - 09673849
KW - chromatin structure
KW - nucleosome positioning
KW - plant telomeres and subtelomeres
N2 - We have analysed the chromatin structure of two subtelomeric tandem repeats from the plant Silene latifolia: X43.1 and 15Ssp. X43.1 shows two distinct nucleosome periodicities - 157 and 188bp. Preferred positions of its two nucleosomes have been mapped at both low and high resolution and the experimental results correspond to computer-redicted positions. 15Ssp is a newly-discovered sequence showing a telomere-associated position by PCR and a subtelomeric location by pulsed-field gel electrophoresis and fluorescence in situ hybridisation. Its 159 bp sequence unit shows a tandem arrangement and the presence of micrococcal nuclease-hypersensitive sites. Use of a chemical nuclease results in a regular nucleosome ladder of 157 bp periodicity. 15Ssp mononucleosomes show instability and absence of specific positioning, features typical for telomeric chromatin.
ER -
SÝKOROVÁ, Eva, Jiří FAJKUS, Ito MIKAKO and Kiichi FUKUI. Transition between two forms of heterochromatin at plant subtelomeres. \textit{Chromosome Research}. Dordrecht: Kluwer Academic Publishers, 2001, roč. 9, January, p.~309-323. ISSN~0967-3849.
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