ŠPANOVÁ, Alena, Bohuslav RITTICH, Renata KARPÍŠKOVÁ, Leona ČECHOVÁ and Denisa ŠKAPOVÁ. PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation. Bioseparation. Nizozemsko: Kluwer Academic Publishers, 2001, vol. 9, No 6, p. 379-384. ISSN 0923-179X. |
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@article{378501, author = {Španová, Alena and Rittich, Bohuslav and Karpíšková, Renata and Čechová, Leona and Škapová, Denisa}, article_location = {Nizozemsko}, article_number = {6}, keywords = {Salmonella; PCR; IMS; inhibitors; food and stool samples}, language = {eng}, issn = {0923-179X}, journal = {Bioseparation}, title = {PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation}, volume = {9}, year = {2001} }
TY - JOUR ID - 378501 AU - Španová, Alena - Rittich, Bohuslav - Karpíšková, Renata - Čechová, Leona - Škapová, Denisa PY - 2001 TI - PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation JF - Bioseparation VL - 9 IS - 6 SP - 379 EP - 379 PB - Kluwer Academic Publishers SN - 0923179X KW - Salmonella KW - PCR KW - IMS KW - inhibitors KW - food and stool samples N2 - The purpose of the present study was to investigate the application of various sample preparation methods (cell washing before lysis, purification of DNA using phenol extraction method, immunomagnetic separation-IMS) for the final PCR identification of Salmonella cells. The presence of PCR inhibitors in processed food products (milk powder and dried eggs) can be the cause of false-negative results in PCR without IMS of target cells. It was also demonstrated that IMS-PCR was succesfully used for identification and quick confirmation of untypical Salmonella strains isolated from human stool samples and rabbit meat. However, IMS cannot eliminate intracellular PCR inhibitors present in immunoseparated Salmonella cells. These inhibitors must be taken into consideration in evaluation of PCR procedure. ER -
ŠPANOVÁ, Alena, Bohuslav RITTICH, Renata KARPÍŠKOVÁ, Leona ČECHOVÁ and Denisa ŠKAPOVÁ. PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation. \textit{Bioseparation}. Nizozemsko: Kluwer Academic Publishers, 2001, vol.~9, No~6, p.~379-384. ISSN~0923-179X.
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