| ŠPANOVÁ, Alena, Bohuslav RITTICH, Renata KARPÍŠKOVÁ, Leona ČECHOVÁ and Denisa ŠKAPOVÁ. PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation. Bioseparation. Nizozemsko: Kluwer Academic Publishers, 2001, vol. 9, No 6, p. 379-384. ISSN 0923-179X. |
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@article{378501,
author = {Španová, Alena and Rittich, Bohuslav and Karpíšková, Renata and Čechová, Leona and Škapová, Denisa},
article_location = {Nizozemsko},
article_number = {6},
keywords = {Salmonella; PCR; IMS; inhibitors; food and stool samples},
language = {eng},
issn = {0923-179X},
journal = {Bioseparation},
title = {PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation},
volume = {9},
year = {2001}
}
TY - JOUR
ID - 378501
AU - Španová, Alena - Rittich, Bohuslav - Karpíšková, Renata - Čechová, Leona - Škapová, Denisa
PY - 2001
TI - PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation
JF - Bioseparation
VL - 9
IS - 6
SP - 379
EP - 379
PB - Kluwer Academic Publishers
SN - 0923179X
KW - Salmonella
KW - PCR
KW - IMS
KW - inhibitors
KW - food and stool samples
N2 - The purpose of the present study was to investigate the application of various sample preparation methods (cell washing before lysis, purification of DNA using phenol extraction method, immunomagnetic separation-IMS) for the final PCR identification of Salmonella cells. The presence of PCR inhibitors in processed food products (milk powder and dried eggs) can be the cause of false-negative results in PCR without IMS of target cells. It was also demonstrated that IMS-PCR was succesfully used for identification and quick confirmation of untypical Salmonella strains isolated from human stool samples and rabbit meat. However, IMS cannot eliminate intracellular PCR inhibitors present in immunoseparated Salmonella cells. These inhibitors must be taken into consideration in evaluation of PCR procedure.
ER -
ŠPANOVÁ, Alena, Bohuslav RITTICH, Renata KARPÍŠKOVÁ, Leona ČECHOVÁ and Denisa ŠKAPOVÁ. PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation. \textit{Bioseparation}. Nizozemsko: Kluwer Academic Publishers, 2001, vol.~9, No~6, p.~379-384. ISSN~0923-179X.
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