Properties of RNase A immobilized on magnetic
poly(2-hydroxyethyl methacrylate) microspheres

J 2001

Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres

HORÁK, Daniel, Bohuslav RITTICH, Jan ŠAFÁŘ, Alena ŠPANOVÁ, Jiří LENFELD et. al.

Basic information

Original name

Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres

Authors

HORÁK, Daniel, Bohuslav RITTICH (203 Czech Republic, guarantor), Jan ŠAFÁŘ, Alena ŠPANOVÁ (203 Czech Republic), Jiří LENFELD and Milan J. BENEŠ

Edition

Biotechnology Progress, U.S.A. Am.Chem. Soc., Washington, 2001, 8756-7938

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Biotechnology and bionics

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.821

RIV identification code

RIV/00216224:14310/01:00004811

Organization unit

Faculty of Science

UT WoS

000169163400009

Keywords in English

Dispersion polymerization; RNase A; nucleic acids; particles; polystyrene; chromatography; purification

Abstract

V originále

Magnetic hydrogel microsheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate and ethylene dimethacrylate in the presence of magnetite, which formed the core of the particles. RNase A was coupled to the particles by the cyanuric chloride method. Gel electrophoresis of plasmid DNA pUC19 (contaminated by bacterial RNA) confirmed RNA degradation with the immobilized enzyme. The effect of temperature and pH on the relative activity of immobilized RNase A was estimated after incubation of the samples at different temperatures (30-80 C) and pH (4.0-8.0). Maximum relative activity was observed at 70 C and pH 6.5. The matrice based on magnetic poly(HEMA) had a low tendency to adsorb RNA.

Links

GA203/00/1339, research and development project

Name: Disperzní polymerizace hydrofilních monometrů pro přípravu magnetických nosičů používaných v biotechnologických aplikacích

Investor: Czech Science Foundation, Dispersion polymerization of hydrofilic monomers for the preparation of magnetic carriers used in biotechnological application

Citovat

HORÁK, Daniel, Bohuslav RITTICH, Jan ŠAFÁŘ, Alena ŠPANOVÁ, Jiří LENFELD and Milan J. BENEŠ. Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres. Biotechnology Progress. U.S.A.: Am.Chem. Soc., Washington, 2001, vol. 17, No 3, p. 447-452. ISSN 8756-7938.

@article{378510,
   author = {Horák, Daniel and Rittich, Bohuslav and Šafář, Jan and Španová, Alena and Lenfeld, Jiří and Beneš, Milan J.},
   article_location = {U.S.A.},
   article_number = {3},
   keywords = {Dispersion polymerization; RNase A; nucleic acids; particles; polystyrene; chromatography; purification},
   language = {eng},
   issn = {8756-7938},
   journal = {Biotechnology Progress},
   title = {Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres},
   volume = {17},
   year = {2001}
}

TY  - JOUR
ID  - 378510
AU  - Horák, Daniel - Rittich, Bohuslav - Šafář, Jan - Španová, Alena - Lenfeld, Jiří - Beneš, Milan J.
PY  - 2001
TI  - Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres
JF  - Biotechnology Progress
VL  - 17
IS  - 3
SP  - 447
EP  - 447
PB  - Am.Chem. Soc., Washington
SN  - 87567938
KW  - Dispersion polymerization
KW  - RNase A
KW  - nucleic acids
KW  - particles
KW  - polystyrene
KW  - chromatography
KW  - purification
N2  - Magnetic hydrogel microsheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate and ethylene dimethacrylate in the presence of magnetite, which formed the core of the particles. RNase A was coupled to the particles by the cyanuric chloride method. Gel electrophoresis of plasmid DNA pUC19 (contaminated by bacterial RNA) confirmed RNA degradation with the immobilized enzyme. The effect of temperature and pH on the relative activity of immobilized RNase A was estimated after incubation of the samples at different temperatures (30-80 C) and pH (4.0-8.0). Maximum relative activity was observed at 70 C and pH 6.5. The matrice based on magnetic poly(HEMA) had a low tendency to adsorb RNA.
ER  -

HORÁK, Daniel, Bohuslav RITTICH, Jan ŠAFÁŘ, Alena ŠPANOVÁ, Jiří LENFELD and Milan J. BENEŠ. Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres. \textit{Biotechnology Progress}. U.S.A.: Am.Chem. Soc., Washington, 2001, vol.~17, No~3, p.~447-452. ISSN~8756-7938.

Detailed Information on Publication Record