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@inproceedings{378519, author = {Španová, Alena and Rittich, Bohuslav and Prodělalová, Jana and Horák, Daniel and Lendfeld, Jiří and Beneš, Milan J.}, address = {Vienna}, booktitle = {Life Sciences 2001}, edition = {2001}, keywords = {Immunomagnetic separation; PCR; Salmonella cells; foods}, language = {eng}, location = {Vienna}, pages = {57-57}, publisher = {Osterreichische Gesselschaft fur Biochemie und Molekularbiologie}, title = {The limitation of PCR in identification of Salmonella cells in foods}, year = {2001} }
TY - JOUR ID - 378519 AU - Španová, Alena - Rittich, Bohuslav - Prodělalová, Jana - Horák, Daniel - Lendfeld, Jiří - Beneš, Milan J. PY - 2001 TI - The limitation of PCR in identification of Salmonella cells in foods PB - Osterreichische Gesselschaft fur Biochemie und Molekularbiologie CY - Vienna KW - Immunomagnetic separation KW - PCR KW - Salmonella cells KW - foods N2 - The aim of the work was to test the utilisation of immunomagnetic separation followed by PCR for the identification of Salmonella cells present in some processed foods containing PCR inhibitors, and of atypically growing Salmonella cells. However, Salmonella cells alone can contain intracellular termostabile inhibitors which must be considered when performing PCR. These inhibitors can be eliminated with free and immobilized proteinase K. ER -
ŠPANOVÁ, Alena, Bohuslav RITTICH, Jana PRODĚLALOVÁ, Daniel HORÁK, Jiří LENDFELD and Milan J. BENEŠ. The limitation of PCR in identification of Salmonella cells in foods. In \textit{Life Sciences 2001}. 2001st ed. Vienna: Osterreichische Gesselschaft fur Biochemie und Molekularbiologie, 2001, p.~57.
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