Influence of metal ions on activity and stability of DNase I
immobilized on hydrophilic particles

D 2001

Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles

RITTICH, Bohuslav, Alena ŠPANOVÁ, Yuriy OHLASHENNYY, Ivo RUDOLF, Daniel HORÁK et. al.

Basic information

Original name

Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles

Authors

RITTICH, Bohuslav (203 Czech Republic, guarantor), Alena ŠPANOVÁ (203 Czech Republic), Yuriy OHLASHENNYY, Ivo RUDOLF (203 Czech Republic), Daniel HORÁK, Jiří LENFELD and Milan J. BENEŠ

Edition

2001. vyd. Zagreb, Croatia, The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists, p. 63-64, 2001

Publisher

Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Croatia

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/01:00004818

Organization unit

Faculty of Science

Keywords in English

immobilization; DNase I; activity; stability; applications

Abstract

V originále

The aim of work has been concentrated on degradation of high-weight and plasmid DNAs using immobilized DNase I. The activity of free and immobilized DNase I was estimated from UV spectrophotometric measurements and from agarose gel electrophoresis. The immobilized enzyme was full actived using Co2+ ions as an activator, a decrease of the activity was observed using other ions. Practical applications of DNase I immobilized on poly(HEMA-co-EDMA) microspheres in cleavage of plasmid DNAs are discussed.

Links

GA203/00/1339, research and development project

Name: Disperzní polymerizace hydrofilních monometrů pro přípravu magnetických nosičů používaných v biotechnologických aplikacích

Investor: Czech Science Foundation, Dispersion polymerization of hydrofilic monomers for the preparation of magnetic carriers used in biotechnological application

Citovat

RITTICH, Bohuslav, Alena ŠPANOVÁ, Yuriy OHLASHENNYY, Ivo RUDOLF, Daniel HORÁK, Jiří LENFELD and Milan J. BENEŠ. Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles. In The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists. 2001st ed. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, p. 63-64.

@inproceedings{378552,
   author = {Rittich, Bohuslav and Španová, Alena and Ohlashennyy, Yuriy and Rudolf, Ivo and Horák, Daniel and Lenfeld, Jiří and Beneš, Milan J.},
   address = {Zagreb, Croatia},
   booktitle = {The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists},
   edition = {2001},
   keywords = {immobilization; DNase I; activity; stability; applications},
   language = {eng},
   location = {Zagreb, Croatia},
   pages = {63-64},
   publisher = {Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia},
   title = {Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles},
   year = {2001}
}

TY  - JOUR
ID  - 378552
AU  - Rittich, Bohuslav - Španová, Alena - Ohlashennyy, Yuriy - Rudolf, Ivo - Horák, Daniel - Lenfeld, Jiří - Beneš, Milan J.
PY  - 2001
TI  - Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles
PB  - Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
CY  - Zagreb, Croatia
KW  - immobilization
KW  - DNase I
KW  - activity
KW  - stability
KW  - applications
N2  - The aim of work has been concentrated on degradation of high-weight and plasmid DNAs using immobilized DNase I. The activity of free and immobilized DNase I was estimated from UV spectrophotometric measurements and from agarose gel electrophoresis. The immobilized enzyme was full actived using Co2+ ions as an activator, a decrease of the activity was observed using other ions. Practical applications of DNase I immobilized on poly(HEMA-co-EDMA) microspheres in cleavage of plasmid DNAs are discussed.
ER  -

RITTICH, Bohuslav, Alena ŠPANOVÁ, Yuriy OHLASHENNYY, Ivo RUDOLF, Daniel HORÁK, Jiří LENFELD and Milan J. BENEŠ. Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles. In \textit{The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists}. 2001st ed. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, p.~63-64.

Detailed Information on Publication Record