RITTICH, Bohuslav, Alena ŠPANOVÁ, Yuriy OHLASHENNYY, Ivo RUDOLF, Daniel HORÁK, Jiří LENFELD and Milan J. BENEŠ. Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles. In The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists. 2001st ed. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, p. 63-64. |
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@inproceedings{378552, author = {Rittich, Bohuslav and Španová, Alena and Ohlashennyy, Yuriy and Rudolf, Ivo and Horák, Daniel and Lenfeld, Jiří and Beneš, Milan J.}, address = {Zagreb, Croatia}, booktitle = {The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists}, edition = {2001}, keywords = {immobilization; DNase I; activity; stability; applications}, language = {eng}, location = {Zagreb, Croatia}, pages = {63-64}, publisher = {Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia}, title = {Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles}, year = {2001} }
TY - JOUR ID - 378552 AU - Rittich, Bohuslav - Španová, Alena - Ohlashennyy, Yuriy - Rudolf, Ivo - Horák, Daniel - Lenfeld, Jiří - Beneš, Milan J. PY - 2001 TI - Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles PB - Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia CY - Zagreb, Croatia KW - immobilization KW - DNase I KW - activity KW - stability KW - applications N2 - The aim of work has been concentrated on degradation of high-weight and plasmid DNAs using immobilized DNase I. The activity of free and immobilized DNase I was estimated from UV spectrophotometric measurements and from agarose gel electrophoresis. The immobilized enzyme was full actived using Co2+ ions as an activator, a decrease of the activity was observed using other ions. Practical applications of DNase I immobilized on poly(HEMA-co-EDMA) microspheres in cleavage of plasmid DNAs are discussed. ER -
RITTICH, Bohuslav, Alena ŠPANOVÁ, Yuriy OHLASHENNYY, Ivo RUDOLF, Daniel HORÁK, Jiří LENFELD and Milan J. BENEŠ. Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles. In \textit{The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists}. 2001st ed. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, p.~63-64.
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