| ŠPANOVÁ, Alena, Bohuslav RITTICH, Jiří LENFELD, Jana PRODĚLALOVÁ, Daniel HORÁK and Milan J. BENEŠ. Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods. In The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists. 2001st ed. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, p. 67-68. |
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@inproceedings{378553,
author = {Španová, Alena and Rittich, Bohuslav and Lenfeld, Jiří and Prodělalová, Jana and Horák, Daniel and Beneš, Milan J.},
address = {Zagreb, Croatia},
booktitle = {The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists},
edition = {2001},
keywords = {Salmonella cells; foods; PCR; preparation of samples; HEMA particles},
language = {eng},
location = {Zagreb, Croatia},
pages = {67-68},
publisher = {Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia},
title = {Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods},
year = {2001}
}
TY - JOUR
ID - 378553
AU - Španová, Alena - Rittich, Bohuslav - Lenfeld, Jiří - Prodělalová, Jana - Horák, Daniel - Beneš, Milan J.
PY - 2001
TI - Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods
PB - Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
CY - Zagreb, Croatia
KW - Salmonella cells
KW - foods
KW - PCR
KW - preparation of samples
KW - HEMA particles
N2 - The quality of food products isalso affected by the microoragnisms contamination during the used technological processes.The aim of the research was to study the elimination of the inhibitor influence on the identification of target microorganisms in real samples using PCR. Salmonella, an important pathogen from hygienic point of wiev, was choosen as a model microorganism The polymerase chain reaction coupled with immunomagnetic separation (IMS-PCR) was used for the elimination of extracellular inhibitors and quick detection of Salmonella cells. The advantage of the use of proteinase K immobilized on poly(HEMA-co-EDMA) microspheres is discussed.
ER -
ŠPANOVÁ, Alena, Bohuslav RITTICH, Jiří LENFELD, Jana PRODĚLALOVÁ, Daniel HORÁK and Milan J. BENEŠ. Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods. In \textit{The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists}. 2001st ed. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, p.~67-68.
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