Application of HEMA-based particles in preparation of samples
for PCR identification of Salmonella cells in foods

D 2001

Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods

ŠPANOVÁ, Alena, Bohuslav RITTICH, Jiří LENFELD, Jana PRODĚLALOVÁ, Daniel HORÁK et. al.

Basic information

Original name

Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods

Authors

ŠPANOVÁ, Alena (203 Czech Republic), Bohuslav RITTICH (203 Czech Republic, guarantor), Jiří LENFELD, Jana PRODĚLALOVÁ (203 Czech Republic), Daniel HORÁK and Milan J. BENEŠ

Edition

2001. vyd. Zagreb, Croatia, The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists, p. 67-68, 2001

Publisher

Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Croatia

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/01:00004819

Organization unit

Faculty of Science

Keywords in English

Salmonella cells; foods; PCR; preparation of samples; HEMA particles

Abstract

V originále

The quality of food products isalso affected by the microoragnisms contamination during the used technological processes.The aim of the research was to study the elimination of the inhibitor influence on the identification of target microorganisms in real samples using PCR. Salmonella, an important pathogen from hygienic point of wiev, was choosen as a model microorganism The polymerase chain reaction coupled with immunomagnetic separation (IMS-PCR) was used for the elimination of extracellular inhibitors and quick detection of Salmonella cells. The advantage of the use of proteinase K immobilized on poly(HEMA-co-EDMA) microspheres is discussed.

Links

GA203/00/1339, research and development project

Name: Disperzní polymerizace hydrofilních monometrů pro přípravu magnetických nosičů používaných v biotechnologických aplikacích

Investor: Czech Science Foundation, Dispersion polymerization of hydrofilic monomers for the preparation of magnetic carriers used in biotechnological application

Citovat

ŠPANOVÁ, Alena, Bohuslav RITTICH, Jiří LENFELD, Jana PRODĚLALOVÁ, Daniel HORÁK and Milan J. BENEŠ. Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods. In The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists. 2001st ed. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, p. 67-68.

@inproceedings{378553,
   author = {Španová, Alena and Rittich, Bohuslav and Lenfeld, Jiří and Prodělalová, Jana and Horák, Daniel and Beneš, Milan J.},
   address = {Zagreb, Croatia},
   booktitle = {The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists},
   edition = {2001},
   keywords = {Salmonella cells; foods; PCR; preparation of samples; HEMA particles},
   language = {eng},
   location = {Zagreb, Croatia},
   pages = {67-68},
   publisher = {Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia},
   title = {Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods},
   year = {2001}
}

TY  - JOUR
ID  - 378553
AU  - Španová, Alena - Rittich, Bohuslav - Lenfeld, Jiří - Prodělalová, Jana - Horák, Daniel - Beneš, Milan J.
PY  - 2001
TI  - Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods
PB  - Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
CY  - Zagreb, Croatia
KW  - Salmonella cells
KW  - foods
KW  - PCR
KW  - preparation of samples
KW  - HEMA particles
N2  - The quality of food products isalso affected by the microoragnisms contamination during the used technological processes.The aim of the research was to study the elimination of the inhibitor influence on the identification of target microorganisms in real samples using PCR. Salmonella, an important pathogen from hygienic point of wiev, was choosen as a model microorganism The polymerase chain reaction coupled with immunomagnetic separation (IMS-PCR) was used for the elimination of extracellular inhibitors and quick detection of Salmonella cells. The advantage of the use of proteinase K immobilized on poly(HEMA-co-EDMA) microspheres is discussed.
ER  -

ŠPANOVÁ, Alena, Bohuslav RITTICH, Jiří LENFELD, Jana PRODĚLALOVÁ, Daniel HORÁK and Milan J. BENEŠ. Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods. In \textit{The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists}. 2001st ed. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, p.~67-68.

Detailed Information on Publication Record