v-Jun and v-Fos proteins induce dedifferentiation of
v-myb-transformed monoblasts

D 2001

v-Jun and v-Fos proteins induce dedifferentiation of v-myb-transformed monoblasts

ŠEVČÍKOVÁ, Sabina, Eva ZAHRADNÍČKOVÁ, Karel SOUČEK and Jan ŠMARDA

Basic information

Original name

v-Jun and v-Fos proteins induce dedifferentiation of v-myb-transformed monoblasts

Authors

ŠEVČÍKOVÁ, Sabina (203 Czech Republic), Eva ZAHRADNÍČKOVÁ (203 Czech Republic), Karel SOUČEK (203 Czech Republic) and Jan ŠMARDA (203 Czech Republic, guarantor)

Edition

Frederick, Maryland, USA, The Seventeenth Annual Meeting on Oncogene, p. 171-171, 2001

Publisher

Foundation for Advanced Cancer Studies, Inc.

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/01:00004846

Organization unit

Faculty of Science

Keywords in English

Myb; Fos; Jun; differentiation

Abstract

V originále

The fos/jun proto-oncogenes encode for proteins which are components of the AP-1 transcription complex that includes several Fos related and Jun related proteins. The AP-1 was shown to play multiple roles in functional development of hematopoietic precursor cells into mature blood cells. Proto-oncogenes of the fos/jun family are considered to be positive regulators of myeloid differentiation. Enforced c-fos and c-jun expression in certain myeloid leukemia cell lines results in increased propensity to differentiate. The line of v-Myb-transformed chicken monoblasts (BM2) can be induced to differentiate phorbol esters. Differentiation-promoting effects of 12-O-tetradecanoyl phorbol 13-acetate (TPA) on BM2 cells is carried though activation of protein kinase C followed by changes of activities of differentiation and proliferation regulators, including AP-1. To gain further insights into the role of fos/jun in leukemogenicity, BM2vJUN and BM2vFOS cell lines, which inducibly express v-jun and v-fos, respectively, were established. We found that either v-fos- or v-jun-expressing BM2 cells exhibit signs of dedifferentiation. They decrease ability to reduce NBT and increase expression of differentiation-specific markers as Mo-1 and MEP-17. This suggests that oncogenic forms of Jun and Fos proteins can further enforce v-Myb transformation.

Links

GA301/01/0040, research and development project

Name: Úloha proteinů Fos a Jun v monocytické diferenciaci stimulované kyselinou retinovou

Investor: Czech Science Foundation, The Role of Fos and Jun proteins in RA-driven monocytic differentiation

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

ŠEVČÍKOVÁ, Sabina, Eva ZAHRADNÍČKOVÁ, Karel SOUČEK and Jan ŠMARDA. v-Jun and v-Fos proteins induce dedifferentiation of v-myb-transformed monoblasts. In The Seventeenth Annual Meeting on Oncogene. Frederick, Maryland, USA: Foundation for Advanced Cancer Studies, Inc., 2001, p. 171.

@inproceedings{378866,
   author = {Ševčíková, Sabina and Zahradníčková, Eva and Souček, Karel and Šmarda, Jan},
   address = {Frederick, Maryland, USA},
   booktitle = {The Seventeenth Annual Meeting on Oncogene},
   keywords = {Myb; Fos; Jun; differentiation},
   language = {eng},
   location = {Frederick, Maryland, USA},
   pages = {171-171},
   publisher = {Foundation for Advanced Cancer Studies, Inc.},
   title = {v-Jun and v-Fos proteins induce dedifferentiation of v-myb-transformed monoblasts},
   year = {2001}
}

TY  - JOUR
ID  - 378866
AU  - Ševčíková, Sabina - Zahradníčková, Eva - Souček, Karel - Šmarda, Jan
PY  - 2001
TI  - v-Jun and v-Fos proteins induce dedifferentiation of v-myb-transformed monoblasts
PB  - Foundation for Advanced Cancer Studies, Inc.
CY  - Frederick, Maryland, USA
KW  - Myb
KW  - Fos
KW  - Jun
KW  - differentiation
N2  - The fos/jun proto-oncogenes encode for proteins which are components of the AP-1 transcription complex that includes several Fos related and Jun related proteins. The AP-1 was shown to play multiple roles in functional development of hematopoietic precursor cells into mature blood cells. Proto-oncogenes of the fos/jun family are considered to be positive regulators of myeloid differentiation. Enforced c-fos and c-jun expression in certain myeloid leukemia cell lines results in increased propensity to differentiate. The line of v-Myb-transformed chicken monoblasts (BM2) can be induced to differentiate phorbol esters. Differentiation-promoting effects of 12-O-tetradecanoyl phorbol 13-acetate (TPA) on BM2 cells is carried though activation of protein kinase C followed by changes of activities of differentiation and proliferation regulators, including AP-1. To gain further insights into the role of fos/jun in leukemogenicity, BM2vJUN and BM2vFOS cell lines, which inducibly express v-jun and v-fos, respectively, were established. We found that either v-fos- or v-jun-expressing BM2 cells exhibit signs of dedifferentiation. They decrease ability to reduce NBT and increase expression of differentiation-specific markers as Mo-1 and MEP-17. This suggests that oncogenic forms of Jun and Fos proteins can further enforce v-Myb transformation.
ER  -

ŠEVČÍKOVÁ, Sabina, Eva ZAHRADNÍČKOVÁ, Karel SOUČEK and Jan ŠMARDA. v-Jun and v-Fos proteins induce dedifferentiation of v-myb-transformed monoblasts. In \textit{The Seventeenth Annual Meeting on Oncogene}. Frederick, Maryland, USA: Foundation for Advanced Cancer Studies, Inc., 2001, p.~171.

Detailed Information on Publication Record