Investigation of highly sensitive piezoelectric immunosensors
for 2,4-dichlorophenoxyacetic acid

J 2001

Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid

HALÁMEK, Jan, Maria HEPEL and Petr SKLÁDAL

Basic information

Original name

Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid

Authors

HALÁMEK, Jan, Maria HEPEL and Petr SKLÁDAL

Edition

Biosensors & Bioelectronics, Oxford, Elsevier Advanced Technology, 2001, 0956-5663

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.714

RIV identification code

RIV/00216224:14310/01:00004853

Organization unit

Faculty of Science

UT WoS

000169712700005

Keywords in English

quartz crystal microbalance; immunosensor; 2;4-dichlorophenoxyacetic acid

Abstract

V originále

The improved highly sensitive piezoelectric immunosensor has been developed and evaluated using a model interaction of antibody with the model hapten-herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). For immobilization of 2,4-D, the self-assembled layers of cystamine, 4-aminothiophenol or 3,3'-dithio-bis(propionic acid N-hydroxysuccinimide ester) were formed on smooth and rough crystals coated with gold or silver electrodes. The immunochemical interactions performed well in all cases, the aminothiophenol on gold was chosen as the optimum with regard to regeneration of immunosensing surfaces. The kinetics of interaction of surface-bound 2,4-D with free antibody provided significantly higher kinetic parameters (kinetic association rate constant) when using optically smooth crystals compared to common rough crystal. Therefore. the smooth crystal should be preferred for future kinetic studies. The competitive assay of the herbicide 2,4-D achieved a limit of detection of 10 ng/l using the monoclonal anti-2,4-D antibody F6C10. Finally, a direct assay format has been evaluated using a thicker layer of glutaraldehyde-crosslinked antibody on the sensing surface. The direct binding of a small herbicide molecule was follon ed in real time. The detected concentration of 2,4-D (5 ug/l) was low enough for future direct monitoring of this herbicide in water.

Links

OC 518.30, research and development project

Name: Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů

Investor: Ministry of Education, Youth and Sports of the CR, Characterization of interactions of biomacromolecules with biolayers in different conformation states using piezoelectric biosensors

Citovat

HALÁMEK, Jan, Maria HEPEL and Petr SKLÁDAL. Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid. Biosensors & Bioelectronics. Oxford: Elsevier Advanced Technology, 2001, vol. 16, No 3, p. 253-260. ISSN 0956-5663.

@article{378954,
   author = {Halámek, Jan and Hepel, Maria and Skládal, Petr},
   article_location = {Oxford},
   article_number = {3},
   keywords = {quartz crystal microbalance; immunosensor; 2;4-dichlorophenoxyacetic acid},
   language = {eng},
   issn = {0956-5663},
   journal = {Biosensors & Bioelectronics},
   title = {Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid},
   volume = {16},
   year = {2001}
}

TY  - JOUR
ID  - 378954
AU  - Halámek, Jan - Hepel, Maria - Skládal, Petr
PY  - 2001
TI  - Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid
JF  - Biosensors & Bioelectronics
VL  - 16
IS  - 3
SP  - 253
EP  - 253
PB  - Elsevier Advanced Technology
SN  - 09565663
KW  - quartz crystal microbalance
KW  - immunosensor
KW  - 2;4-dichlorophenoxyacetic acid
N2  - The improved highly sensitive piezoelectric immunosensor has been developed and evaluated using a model interaction of antibody with the model hapten-herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). For immobilization of 2,4-D, the self-assembled layers of cystamine, 4-aminothiophenol or 3,3'-dithio-bis(propionic acid N-hydroxysuccinimide ester) were formed on smooth and rough crystals coated with gold or silver electrodes. The immunochemical interactions performed well in all cases, the aminothiophenol on gold was chosen as the optimum with regard to regeneration of immunosensing surfaces. The kinetics of interaction of surface-bound 2,4-D with free antibody provided significantly higher kinetic parameters (kinetic association rate constant) when using optically smooth crystals compared to common rough crystal. Therefore. the smooth crystal should be preferred for future kinetic studies. The competitive assay of the herbicide 2,4-D achieved a limit of detection of 10 ng/l using the monoclonal anti-2,4-D antibody F6C10. Finally, a direct assay format has been evaluated using a thicker layer of glutaraldehyde-crosslinked antibody on the sensing surface. The direct binding of a small herbicide molecule was follon ed in real time. The detected concentration of 2,4-D (5 ug/l) was low enough for future direct monitoring of this herbicide in water.
ER  -

HALÁMEK, Jan, Maria HEPEL and Petr SKLÁDAL. Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid. \textit{Biosensors \&{} Bioelectronics}. Oxford: Elsevier Advanced Technology, 2001, vol.~16, No~3, p.~253-260. ISSN~0956-5663.

Detailed Information on Publication Record