Detailed Information on Publication Record
2001
Development of piezoelectric immunosensors for measurement of albuminuria
NAVRÁTILOVÁ, Iva, Petr SKLÁDAL and Vladimír VIKLICKÝBasic information
Original name
Development of piezoelectric immunosensors for measurement of albuminuria
Authors
NAVRÁTILOVÁ, Iva, Petr SKLÁDAL and Vladimír VIKLICKÝ
Edition
Talanta, Elsevier Science, 2001, 0039-9140
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.587
RIV identification code
RIV/00216224:14310/01:00004854
Organization unit
Faculty of Science
UT WoS
000173288900020
Keywords in English
Immunosensor; Quartz crystal microbalance; Human serum albumin; Albuminuria
Změněno: 5/12/2001 10:35, prof. RNDr. Petr Skládal, CSc.
Abstract
V originále
The development of piezoelectric immunosensors for human serum albumin (HSA) is reported. The piezoelectric crystals were modified either with monoclonal antibody AL-01 (direct assay) or with HSA (competitive assay). Measurements were carried out in the flow-through mode. Affinity interaction between albumin and the antibody was characterised. With immobilised antibody and HSA in solution, the kinetic association rate constant ka was 18100 l mol-1 s-1 and the dissociation constant kd was 0.00369 s-1. For the opposite arrangement (immobilised HSA), a slower dissociation was observed, kd was 0.00085 s-1. A competitive assay for HSA was developed with working range of 1 to 5 ug/ml and a total time for one analysis equal to 17 min. Samples of urine were analysed after tenfold dilution. The developed system was successfully evaluated on real samples from diabetic patients and the obtained results correlated well with the standard reflectometric assay of proteins in urine.
Links
| OC 518.30, research and development project |
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