NAVRÁTILOVÁ, Iva, Petr SKLÁDAL and Vladimír VIKLICKÝ. Development of piezoelectric immunosensors for measurement of albuminuria. Talanta. Elsevier Science, 2001, vol. 55, No 11, p. 831-839. ISSN 0039-9140. |
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@article{378971, author = {Navrátilová, Iva and Skládal, Petr and Viklický, Vladimír}, article_number = {11}, keywords = {Immunosensor; Quartz crystal microbalance; Human serum albumin; Albuminuria}, language = {eng}, issn = {0039-9140}, journal = {Talanta}, title = {Development of piezoelectric immunosensors for measurement of albuminuria}, volume = {55}, year = {2001} }
TY - JOUR ID - 378971 AU - Navrátilová, Iva - Skládal, Petr - Viklický, Vladimír PY - 2001 TI - Development of piezoelectric immunosensors for measurement of albuminuria JF - Talanta VL - 55 IS - 11 SP - 831 EP - 831 PB - Elsevier Science SN - 00399140 KW - Immunosensor KW - Quartz crystal microbalance KW - Human serum albumin KW - Albuminuria N2 - The development of piezoelectric immunosensors for human serum albumin (HSA) is reported. The piezoelectric crystals were modified either with monoclonal antibody AL-01 (direct assay) or with HSA (competitive assay). Measurements were carried out in the flow-through mode. Affinity interaction between albumin and the antibody was characterised. With immobilised antibody and HSA in solution, the kinetic association rate constant ka was 18100 l mol-1 s-1 and the dissociation constant kd was 0.00369 s-1. For the opposite arrangement (immobilised HSA), a slower dissociation was observed, kd was 0.00085 s-1. A competitive assay for HSA was developed with working range of 1 to 5 ug/ml and a total time for one analysis equal to 17 min. Samples of urine were analysed after tenfold dilution. The developed system was successfully evaluated on real samples from diabetic patients and the obtained results correlated well with the standard reflectometric assay of proteins in urine. ER -
NAVRÁTILOVÁ, Iva, Petr SKLÁDAL and Vladimír VIKLICKÝ. Development of piezoelectric immunosensors for measurement of albuminuria. \textit{Talanta}. Elsevier Science, 2001, vol.~55, No~11, p.~831-839. ISSN~0039-9140.
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