Detailed Information on Publication Record
2001
Application of Capillary Zone Electrophoresis to Study the Properties of Rhodanese from Acidithiobacillus ferrooxidans
BOUCHAL, Pavel, Zdeněk GLATZ, Oldřich JANICZEK and Martin MANDLBasic information
Original name
Application of Capillary Zone Electrophoresis to Study the Properties of Rhodanese from Acidithiobacillus ferrooxidans
Authors
BOUCHAL, Pavel (203 Czech Republic), Zdeněk GLATZ (203 Czech Republic), Oldřich JANICZEK (203 Czech Republic) and Martin MANDL (203 Czech Republic)
Edition
Folia Microbiologica, Praha, Institute of Microbiology of AV ČR, 2001, 0015-5632
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.776
RIV identification code
RIV/00216224:14310/01:00005041
Organization unit
Faculty of Science
UT WoS
000173438200005
Keywords in English
rhodanese; CZE
Změněno: 9/12/2004 18:18, doc. Ing. Martin Mandl, CSc.
Abstract
V originále
The new capillary zone electrophoretic method was applied for the assay of the enzymatic activity of the rhodanese from Acidithiobacillus ferrooxidans. The enzyme activities determined by capillary zone electrophoresis were compared with those determined by discontinuous spectrophotometric method. The values obtained were in a good agreement. The method was also used to evaluate Michaelis constants for both substrates; a new approach was developed to solve the problem with variable ionic strength of the samples. The other properties of the enzyme such as the pH and temperature optima were determined as well.
Links
| GA525/00/0785, research and development project |
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| MSM 143100005, plan (intention) |
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