Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae

SVOBODA, Augustin, Iva SLANINOVÁ and Alena HOLUBÁŘOVÁ. Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae. Acta Biologica Hungarica. Budapest: Akademiai Kiado, 2001, vol. 52, No 2, p. 325-332. ISSN 0236-5383-01.

Basic information

• Original name: Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae
• Authors: SVOBODA, Augustin, Iva SLANINOVÁ and Alena HOLUBÁŘOVÁ.
• Edition: Acta Biologica Hungarica, Budapest, Akademiai Kiado, 2001, 0236-5383-01.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Hungary
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/01:00005112
• Organization unit: Faculty of Medicine
• UT WoS: 000169642400017
• Keywords in English: yeast; protoplasts; cell wall; actin; microtubules; cell polarity
• Tags: Actin, cell polarity, Cell wall, Microtubules, protoplasts, Yeast

Abstract

The actin cables and microtubules were disrupted during protoplasting in Saccharomyces cerevisiae cells. In the process of protoplast regeneration, the cytoplasmic microtubules were the first to be restored; actin patches remained regularly distributed under the surface of growing protoplast. After the cell wall had been regenerated in a gelatine medium, actin patches aggregated into clusters, which marked the site of bud development. An incomplete cell wall was the apparent cause for uncoupling between karyokinesis and cytokinesis The presence of several nuclei in the cell gave rise to several buds emerging simultaneously and was probably related to their random positions. In haploids, however, the axial type of budding was obvious also in the reverting protoplasts. Our observations suggest that actin is a structure involved in the restoration of polar growth during protoplast regeneration and that the cell wall plays an important role in this process: in its absence, actin fails to polarise.

Links

• GA204/00/0394, research and development project: Name: Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

Investor: Czech Science Foundation, The yeast cell wall as an extracellular matrix: cytoskeleton and cell wall functions

• GA204/99/D025, research and development project: Name: Úloha cytoskeletu a buněčné stěny v polarizaci buňky

Investor: Czech Science Foundation, The role of cytoskeleton and cell wall in the cell polarization