| PREISLER, Jan, Ping HU, Barry, L. KARGER, Tomáš REJTAR and Eugene MOSKOVEETS. Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2002, vol. 74, No 1, p. 17-25. ISSN 0003-2700. |
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@article{384181,
author = {Preisler, Jan and Hu, Ping and Karger, Barry, L. and Rejtar, Tomáš and Moskoveets, Eugene},
article_location = {Washington, D.C., USA},
article_number = {1},
keywords = {capillary array electrophoresis; MALDI; mass spectrometry; proteomics; peptide},
language = {eng},
issn = {0003-2700},
journal = {Analytical Chemistry},
title = {Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface},
volume = {74},
year = {2002}
}
TY - JOUR
ID - 384181
AU - Preisler, Jan - Hu, Ping - Karger, Barry, L. - Rejtar, Tomáš - Moskoveets, Eugene
PY - 2002
TI - Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface
JF - Analytical Chemistry
VL - 74
IS - 1
SP - 17
EP - 17
PB - American Chemical Society
SN - 00032700
KW - capillary array electrophoresis
KW - MALDI
KW - mass spectrometry
KW - proteomics
KW - peptide
N2 - The approach of vacuum deposition has been extended to demonstrate parallel analysis for multiple on-line infusion MALDI TOF MS and capillary array electrophoresis (CAE)-MALDI MS. In the infusion mode, individual peptide samples were simultaneously deposited on a Mylar tape cartridge using an array of eight capillaries, yielding eight parallel traces. For CAE-MALDI/TOF MS, the same number of separation capillaries were coupled with an array of eight infusion capillaries using a common liquid junction, containing matrix solution. A fast-scanning mirror was employed to traverse the beam of the desorption laser across the Mylar tape to probe one trace at a time. The positions of the eight sample traces formed on the tape were automatically determined, and all samples were analyzed in rapid sequence using a kilohertz repetition rate laser and a high-throughput data acquisition system. The instrumentation was operated with CAE MS for high-throughput analysis without compromising data quality applicable to MALDI/TOF MS analysis for proteomics and other areas where separation and high throughput are required.
ER -
PREISLER, Jan, Ping HU, Barry, L. KARGER, Tomáš REJTAR and Eugene MOSKOVEETS. Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2002, vol.~74, No~1, p.~17-25. ISSN~0003-2700.
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