Ultracytochemical identification of hydrogen peroxide in inseminated human cumuli oophori

SEDLÁČKOVÁ, Miroslava, Jitka ŠŤASTNÁ, Jana ŽÁKOVÁ and Pavel VENTRUBA. Ultracytochemical identification of hydrogen peroxide in inseminated human cumuli oophori. Sborník lékařský. Praha: Karolinum, 2001, vol. 102, No 2, p. 301. ISSN 0036-5327.

Basic information

• Original name: Ultracytochemical identification of hydrogen peroxide in inseminated human cumuli oophori
• Authors: SEDLÁČKOVÁ, Miroslava (203 Czech Republic), Jitka ŠŤASTNÁ (203 Czech Republic), Jana ŽÁKOVÁ (203 Czech Republic) and Pavel VENTRUBA (203 Czech Republic).
• Edition: Sborník lékařský, Praha, Karolinum, 2001, 0036-5327.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30214 Obstetrics and gynaecology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/01:00005302
• Organization unit: Faculty of Medicine
• Keywords in English: hydrogen peroxide; ultracytochemistry; cumulus oophorus; spermatozoa; human
• Tags: cumulus oophorus, human, hydrogen peroxide, spermatozoa, ultracytochemistry

Abstract

The hydrogen peroxide generation was detected in cumulus oophorus cells 2 h after insemination in human in vitro ferttilisation programme. Spermatozoa of both normal and abnormal morphology produced excessive amounts of hydrogen peroxide. Damaged cumulus cells are an additional source of this metabolite.

Links

• NH5164, research and development project: Name: Identifikace peroxisomů a jejich úloha v eliminaci poškození lidských zárodečných buněk kyslíkovými radikály při in vitro fertilizaci

Investor: Ministry of Health of the CR, Identification of Peroxisomes and Their Role in Germinal Cells Protection From Free Oxygen Radicals in Human In Vitro Fertilization