This paper presents a survey of the pleurotoid genera belonging to the family Polyporaceae in the Czech Republic and Slovakia. It is based on material deposited in Czech and Slovak herbaria as well as on published records of finds of the included species from the studied territory. For each species a short description is provided, accompanied by taxonomic or nomenclatoric notes in some problematic cases, and characters distinguishing it from related species are highlighted. Short notes about phenology, ecology, occurrence and distribution are included. The study is supplemented with distribution maps for individual species. A new combination, Neolentinus degener, is submitted instead of Neolentinus cyathiformis (Schaeff.: Fr.) Redhead et Ginns.