| KIZEK, René, Libuše TRNKOVÁ, Sabina ŠEVČÍKOVÁ, Jan ŠMARDA a František JELEN. Silver Electrode as a Sensor for Determination of Zinc in Cell Cultivation Medium. Analytical Biochemistry. San Diego: Academic Press, 2002, roč. 301, č. 2002, s. 8-13. ISSN 0003-2697. |
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@article{390991,
author = {Kizek, René and Trnková, Libuše and Ševčíková, Sabina and Šmarda, Jan and Jelen, František},
article_location = {San Diego},
article_number = {2002},
keywords = {silver electrode; cyclic voltammetry; zinc in growth medium; inducible gene expression; BM2 cells; immunoblotting},
language = {eng},
issn = {0003-2697},
journal = {Analytical Biochemistry},
title = {Silver Electrode as a Sensor for Determination of Zinc in Cell Cultivation Medium},
volume = {301},
year = {2002}
}
TY - JOUR
ID - 390991
AU - Kizek, René - Trnková, Libuše - Ševčíková, Sabina - Šmarda, Jan - Jelen, František
PY - 2002
TI - Silver Electrode as a Sensor for Determination of Zinc in Cell Cultivation Medium
JF - Analytical Biochemistry
VL - 301
IS - 2002
SP - 8
EP - 8
PB - Academic Press
SN - 00032697
KW - silver electrode
KW - cyclic voltammetry
KW - zinc in growth medium
KW - inducible gene expression
KW - BM2 cells
KW - immunoblotting
N2 - Use of the silver electrode as a sensor for monitoring of zinc in cell growth medium is described. Zinc at siver electrodes provides specific voltammetric signal, which is affected by solution components. Signlas of zinc ions in phosphate buffer solutions with and without cell growth meidum were compared. Common DMEM cell culture medium was used for the cultivation of a cell line of v-myb-transformed chicken monoblasts and its variants expressing v-jun and c-jun in a zinc dependent manner. Electrochemical results showed zinc concentrations in the medium coincide very well with the jun expression. With respect to the low toxicity of xilver for eukaryotic cells, silver electrodes represent promising tools for the determination of zinc concentrations in vivo without the potential risk of a cell culture damage.
ER -
KIZEK, René, Libuše TRNKOVÁ, Sabina ŠEVČÍKOVÁ, Jan ŠMARDA a František JELEN. Silver Electrode as a Sensor for Determination of Zinc in Cell Cultivation Medium. \textit{Analytical Biochemistry}. San Diego: Academic Press, 2002, roč.~301, č.~2002, s.~8-13. ISSN~0003-2697.
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