KOZUBEK, Michal, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Eva BÁRTOVÁ, Magdalena SKALNÍKOVÁ, Pavel MATULA, Petr MATULA, Pavla JIRSOVÁ, Alena GAŇOVÁ and Irena KOUTNÁ. Advances in high-resolution cytometry of FISH dots in interphase cell nuclei. In The XX Congress of the International Society for Analytical Cytology. 2000.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Advances in high-resolution cytometry of FISH dots in interphase cell nuclei
Authors KOZUBEK, Michal (203 Czech Republic, guarantor), Stanislav KOZUBEK (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Magdalena SKALNÍKOVÁ (203 Czech Republic), Pavel MATULA (203 Czech Republic), Petr MATULA (203 Czech Republic), Pavla JIRSOVÁ (203 Czech Republic), Alena GAŇOVÁ (203 Czech Republic) and Irena KOUTNÁ (203 Czech Republic).
Edition The XX Congress of the International Society for Analytical Cytology, 2000.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 20200 2.2 Electrical engineering, Electronic engineering, Information engineering
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14330/00:00005455
Organization unit Faculty of Informatics
Keywords in English automated microscopy; image analysis; high-resolution cytometry; fluorescence in situ hybridization; interphase nuclei; 3-D analysis
Tags 3-D analysis, automated microscopy, fluorescence in situ hybridization, high-resolution cytometry, Image analysis, interphase nuclei
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Michal Kozubek, Ph.D., učo 3740. Changed: 7/5/2010 16:58.
Abstract
Recently, we have described a high-resolution cytometry (HRCM) technique (Cytometry 36:279-293) as a reasonable compromise between flow cytometry (FCM) and laser scanning cytometry (LSCM) on one hand and confocal laser scanning microscopy (CLSM) on the other hand. Whereas FCM/LSCM and CLSM enable measuring of either large number of cells with low resolution or small number of cells with high resolution, respectively, HRCM can analyze quantities comparable to FCM/LSCM measurements (using overnight acquisition) with an accuracy comparable to CLSM. Very recently we have built a second HRCM instrument (see picture at http://www.fi.muni.cz/lom) which enables switching between conventional and confocal modes. The confocal mode is realized using a 5% Nipkow disk optimized for fluorescence imaging. It offers a better resolution but at a lower speed. The user can choose which fluorochromes are imaged in confocal mode. Thus, the system can be configured for a variety of imaging modes ranging from quick 2-D analysis up to high-resolution confocal 3-D studies.
Links
MSM 143300002, plan (intention)Name: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
PrintDisplayed: 29/3/2024 09:09