Advances in high-resolution cytometry of FISH dots in
interphase cell nuclei

a 2000

Advances in high-resolution cytometry of FISH dots in interphase cell nuclei

KOZUBEK, Michal, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Eva BÁRTOVÁ, Magdalena SKALNÍKOVÁ et. al.

Basic information

Original name

Advances in high-resolution cytometry of FISH dots in interphase cell nuclei

Authors

KOZUBEK, Michal (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Magdalena SKALNÍKOVÁ (203 Czech Republic), Pavel MATULA (203 Czech Republic), Petr MATULA (203 Czech Republic), Pavla JIRSOVÁ (203 Czech Republic), Alena GAŇOVÁ (203 Czech Republic) and Irena KOUTNÁ (203 Czech Republic)

Edition

Cytokinematics 2000, 2000

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14330/00:00005457

Organization unit

Faculty of Informatics

Keywords in English

automated microscopy; image analysis; high-resolution cytometry; fluorescence in situ hybridization; interphase nuclei; 3-D analysis

Abstract

V originále

Recently, we have described a high-resolution cytometry (HRCM) technique (Cytometry 36:279-293) as a reasonable compromise between flow cytometry (FCM) and laser scanning cytometry (LSCM) on one hand and confocal laser scanning microscopy (CLSM) on the other hand. Whereas FCM/LSCM and CLSM enable measuring of either large number of cells with low resolution or small number of cells with high resolution, respectively, HRCM can analyze quantities comparable to FCM/LSCM measurements (using overnight acquisition) with an accuracy comparable to CLSM. Very recently we have built a second HRCM instrument (see picture at http://www.fi.muni.cz/lom) which enables switching between conventional and confocal modes. The confocal mode is realized using a 5% Nipkow disk optimized for fluorescence imaging. It offers a better resolution but at a lower speed. The user can choose which fluorochromes are imaged in confocal mode. Thus, the system can be configured for a variety of imaging modes ranging from quick 2-D analysis up to high-resolution confocal 3-D studies.

Links

MSM 143300002, plan (intention)

Name: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy

Citovat

KOZUBEK, Michal, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Eva BÁRTOVÁ, Magdalena SKALNÍKOVÁ, Pavel MATULA, Petr MATULA, Pavla JIRSOVÁ, Alena GAŇOVÁ and Irena KOUTNÁ. Advances in high-resolution cytometry of FISH dots in interphase cell nuclei. In Cytokinematics 2000. 2000.

@proceedings{391792,
   author = {Kozubek, Michal and Kozubek, Stanislav and Lukášová, Emilie and Bártová, Eva and Skalníková, Magdalena and Matula, Pavel and Matula, Petr and Jirsová, Pavla and Gaňová, Alena and Koutná, Irena},
   booktitle = {Cytokinematics 2000},
   keywords = {automated microscopy; image analysis; high-resolution cytometry; fluorescence in situ hybridization; interphase nuclei; 3-D analysis},
   language = {eng},
   title = {Advances in high-resolution cytometry of FISH dots in interphase cell nuclei},
   year = {2000}
}

TY  - CONF
ID  - 391792
AU  - Kozubek, Michal - Kozubek, Stanislav - Lukášová, Emilie - Bártová, Eva - Skalníková, Magdalena - Matula, Pavel - Matula, Petr - Jirsová, Pavla - Gaňová, Alena - Koutná, Irena
PY  - 2000
TI  - Advances in high-resolution cytometry of FISH dots in interphase cell nuclei
KW  - automated microscopy
KW  - image analysis
KW  - high-resolution cytometry
KW  - fluorescence in situ hybridization
KW  - interphase nuclei
KW  - 3-D analysis
N2  - Recently, we have described a high-resolution cytometry (HRCM) technique (Cytometry 36:279-293) as a reasonable compromise between flow cytometry (FCM) and laser scanning cytometry (LSCM) on one hand and confocal laser scanning microscopy (CLSM) on the other hand. Whereas FCM/LSCM and CLSM enable measuring of either large number of cells with low resolution or small number of cells with high resolution, respectively, HRCM can analyze quantities comparable to FCM/LSCM measurements (using overnight acquisition) with an accuracy comparable to CLSM. Very recently we have built a second HRCM instrument (see picture at http://www.fi.muni.cz/lom) which enables switching between conventional and confocal modes. The confocal mode is realized using a 5% Nipkow disk optimized for fluorescence imaging. It offers a better resolution but at a lower speed. The user can choose which fluorochromes are imaged in confocal mode. Thus, the system can be configured for a variety of imaging modes ranging from quick 2-D analysis up to high-resolution confocal 3-D studies.
ER  -

Detailed Information on Publication Record