Western blot determination of IgG avidity in primary and secondary syphilis.

WOZNICOVÁ, Vladana and Miroslav VOTAVA. Western blot determination of IgG avidity in primary and secondary syphilis. Scripta medica (Brno). Brno: MU Brno, 2001, vol. 74, No 5, p. 355-362. ISSN 1211-3395.

Basic information

Original name

Western blot determination of IgG avidity in primary and secondary syphilis.

Name in Czech

Stanovení avidity IgG pomocí westernblotu u případů primární a sekundární syfilidy

Name (in English)

Western blot determination of IgG avidity in primary and secondary syphilis.

Authors

WOZNICOVÁ, Vladana (203 Czech Republic, guarantor) and Miroslav VOTAVA (203 Czech Republic)

Edition

Scripta medica (Brno), Brno, MU Brno, 2001, 1211-3395

---

Other information

Language

Czech

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/01:00030459

Organization unit

Faculty of Medicine

Keywords in English

low-avidity IgG; syphilis; Western blot

Tags

low-avidity IgG, syphilis, Western blot

---

Abstract

V originále

IgG avidity to p15.5 kD, p17 kD, and p47 kD antigens of Treponema pallidum was analysed by a new avidity assay termed avidity Western blot. Group I consisted of sera from 27 patients with primary and secondary syphilis, group II comprised 10 subjects with latent syphilis. Low-avidity antibodies were directed against the p17 kD and p47 kD antigens in group I and against the p47 kD antigen in group II. No low-avidity IgG antibodies were detected by ELISA, a routine method for avidity assessment.

In English

IgG avidity to p15.5 kD, p17 kD, and p47 kD antigens of Treponema pallidum was analysed by a new avidity assay termed avidity Western blot. Group I consisted of sera from 27 patients with primary and secondary syphilis, group II comprised 10 subjects with latent syphilis. Low-avidity antibodies were directed against the p17 kD and p47 kD antigens in group I and against the p47 kD antigen in group II. No low-avidity IgG antibodies were detected by ELISA, a routine method for avidity assessment.