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@article{399251, author = {Glatz, Zdeněk and Ševčíková, Petra}, article_location = {Amstredam}, article_number = {1-2}, keywords = {DTT; MEKC; on-column;}, language = {eng}, issn = {0378-4347}, journal = {Journal of Chromatography B}, title = {Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography}, volume = {770}, year = {2002} }
TY - JOUR ID - 399251 AU - Glatz, Zdeněk - Ševčíková, Petra PY - 2002 TI - Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography JF - Journal of Chromatography B VL - 770 IS - 1-2 SP - 237 EP - 237 PB - Elsevier Science SN - 03784347 KW - DTT KW - MEKC KW - on-column; N2 - A new method for specific determination of dithiothreitol (DTT) using micellar electrokinetic chromatography and on-column reaction with reactive disulfide - 2,2-dipyridyldisulfide is described. DTT is in this reaction quantitatively transformed into a mixed disulfide concomitantly with formation of equimolar amount of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of DTT is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 2.5 mM (correlation coefficient 0.993) with a detection limit of 2.5 mM. The inter-day reproducibility of the peak area was 1.35 % and the inter-day reproducibility of the migration time 0.56 %. The method can be applied for DTT monitoring both in chemical and biological systems. ER -
GLATZ, Zdeněk a Petra ŠEVČÍKOVÁ. Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography. \textit{Journal of Chromatography B}. Amstredam: Elsevier Science, 2002, roč.~770, 1-2, s.~237-241. ISSN~0378-4347.
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