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@article{399251,
author = {Glatz, Zdeněk and Ševčíková, Petra},
article_location = {Amstredam},
article_number = {1-2},
keywords = {DTT; MEKC; on-column;},
language = {eng},
issn = {0378-4347},
journal = {Journal of Chromatography B},
title = {Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography},
volume = {770},
year = {2002}
}
TY - JOUR
ID - 399251
AU - Glatz, Zdeněk - Ševčíková, Petra
PY - 2002
TI - Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography
JF - Journal of Chromatography B
VL - 770
IS - 1-2
SP - 237
EP - 237
PB - Elsevier Science
SN - 03784347
KW - DTT
KW - MEKC
KW - on-column;
N2 - A new method for specific determination of dithiothreitol (DTT) using micellar electrokinetic chromatography and on-column reaction with reactive disulfide - 2,2-dipyridyldisulfide is described. DTT is in this reaction quantitatively transformed into a mixed disulfide concomitantly with formation of equimolar amount of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of DTT is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 2.5 mM (correlation coefficient 0.993) with a detection limit of 2.5 mM. The inter-day reproducibility of the peak area was 1.35 % and the inter-day reproducibility of the migration time 0.56 %. The method can be applied for DTT monitoring both in chemical and biological systems.
ER -
GLATZ, Zdeněk and Petra ŠEVČÍKOVÁ. Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography. \textit{Journal of Chromatography B}. Amstredam: Elsevier Science, 2002, vol.~770, 1-2, p.~237-241. ISSN~0378-4347.
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