J 2002

Electrochemical behavior of DNA at a silver electrode studied by cyclic and elimination voltammetry

TRNKOVÁ, Libuše

Basic information

Original name

Electrochemical behavior of DNA at a silver electrode studied by cyclic and elimination voltammetry

Authors

TRNKOVÁ, Libuše (203 Czech Republic, guarantor)

Edition

Talanta, The Netherlands, Elsevier Science, 2002, 0039-9140

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.054

RIV identification code

RIV/00216224:14310/02:00005593

Organization unit

Faculty of Science

UT WoS

000174977700011

Keywords in English

Silver electrode;DNA;electrochemical biosensor;elimination voltammetry

Tags

Změněno: 30/5/2003 18:43, prof. RNDr. Libuše Trnková, CSc.

Abstract

V originále

Electrochemical characteristics of native and denatured calf thymus DNA have been studied by voltammetry on a silver electrode (AgE). Experimental results obtained from linear sweep or cyclic voltammetry (LSV or CV) have been employed in elimination voltammetry. The best results have been obtained by using a function eliminating the kinetic and charging currents , and conserving the diffusion current . This function makes it possible to increase significantly voltammetric signals of native and denatured DNAs, and to reveal processes not detectable by conventional electrochemical methods. The influence of electrochemical pretreatment of silver electrode surfaces, and of starting and switching potentials on DNA voltammetric signals have been discussed. Silver electrodes coupled with elimination voltammetry represent promising tools for developing new nucleic acids biosensors.

Links

GA203/02/0422, research and development project
Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí
Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry
GV204/97/K084, research and development project
Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu
Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research
IAA1163201, research and development project
Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin
Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.
MSM 143100011, plan (intention)
Name: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů
Investor: Ministry of Education, Youth and Sports of the CR, Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles