Electrochemical behavior of DNA at a silver electrode studied by cyclic and elimination voltammetry

TRNKOVÁ, Libuše. Electrochemical behavior of DNA at a silver electrode studied by cyclic and elimination voltammetry. Talanta. The Netherlands: Elsevier Science, vol. 56, No 56, p. 887-894. ISSN 0039-9140. 2002.

Basic information

• Original name: Electrochemical behavior of DNA at a silver electrode studied by cyclic and elimination voltammetry
• Authors: TRNKOVÁ, Libuše (203 Czech Republic, guarantor).
• Edition: Talanta, The Netherlands, Elsevier Science, 2002, 0039-9140.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10405 Electrochemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 2.054
• RIV identification code: RIV/00216224:14310/02:00005593
• Organization unit: Faculty of Science
• UT WoS: 000174977700011
• Keywords in English: Silver electrode;DNA;electrochemical biosensor;elimination voltammetry
• Tags: DNA, electrochemical biosensor, Elimination voltammetry, silver electrode

Abstract

Electrochemical characteristics of native and denatured calf thymus DNA have been studied by voltammetry on a silver electrode (AgE). Experimental results obtained from linear sweep or cyclic voltammetry (LSV or CV) have been employed in elimination voltammetry. The best results have been obtained by using a function eliminating the kinetic and charging currents , and conserving the diffusion current . This function makes it possible to increase significantly voltammetric signals of native and denatured DNAs, and to reveal processes not detectable by conventional electrochemical methods. The influence of electrochemical pretreatment of silver electrode surfaces, and of starting and switching potentials on DNA voltammetric signals have been discussed. Silver electrodes coupled with elimination voltammetry represent promising tools for developing new nucleic acids biosensors.

Links

• GA203/02/0422, research and development project: Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry

• GV204/97/K084, research and development project: Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research

• IAA1163201, research and development project: Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.

• MSM 143100011, plan (intention): Name: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů

Investor: Ministry of Education, Youth and Sports of the CR, Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles