Prevalence of endothelial nitric oxide synthase gene
polymorphisms in patients with atopic asthma

J 2002

Prevalence of endothelial nitric oxide synthase gene polymorphisms in patients with atopic asthma

IZAKOVIČOVÁ HOLLÁ, Lydie, Dana BUČKOVÁ, Viera KUHROVÁ, Andrea STEJSKALOVÁ, Hana FRANCOVÁ et. al.

Basic information

Original name

Prevalence of endothelial nitric oxide synthase gene polymorphisms in patients with atopic asthma

Authors

IZAKOVIČOVÁ HOLLÁ, Lydie (203 Czech Republic, guarantor), Dana BUČKOVÁ (203 Czech Republic), Viera KUHROVÁ (203 Czech Republic), Andrea STEJSKALOVÁ (203 Czech Republic), Hana FRANCOVÁ (203 Czech Republic), Vladimír ZNOJIL (203 Czech Republic) and Jirí VÁCHA (203 Czech Republic)

Edition

Clin Exp Allergy, England, Blackwell Science, 2002, 0954-7894

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.721

RIV identification code

RIV/00216224:14110/02:00005702

Organization unit

Faculty of Medicine

UT WoS

000177506100014

Keywords in English

nitric oxide synthase; gene; polymorphism; asthma; allergic disease

Abstract

V originále

Asthma is a common multifactorial disease, the etiology of which is attributable to both environmental and genetic factors. The endothelial nitric oxide synthase (NOS3) gene has been implicated in asthma pathogenesis.This study investigated associations of 27 base-pair tandem repeat polymorphism in intron 4 and the Glu298Asp (G894T) variant of the NOS3 gene with atopic asthma in a Czech population. Polymerase chain reaction was used to determine the NOS3 genotypes in subjects with atopic asthma (n=163) and random controls (n=209). The NOS3 allele or genotype distributions did not significantly differ between the control and asthma groups. However, the common genotype (bb) of the NOS3 polymorphism in intron 4 was found to be significantly associated with total IgE levels (P=0.006), specific IgE levels for feathers (p=0.0002) and a positive skin prick test for hay (P=0.004). In one atopic patient, we identified an additional 27-bp repeat in the NOS3 gene (NOS3c), which occurred in heterozygous combination with the NOS3b allele (NOS3b/c genotype). In addition, we describe a new polymorphism (A5495G) in the NOS3 gene, which was in almost complete linkage disequilibrium with the NOS3 repeat polymorphism in intron 4. The Glu298Asp variant was not associated with asthma and/or related atopic phenotypes in our study. Neither the NOS3 b allele nor the NOS3 b/b genotype showed any general association with atopic asthma, but they were associated with atopy-related phenotypes. We conclude that the NOS3 gene polymorphisms may act as disease modifiers in atopic asthma phenotype in our population.

Links

MSM 141100002, plan (intention)

Name: Molekulární patofyziologie multigenních chorob

Investor: Ministry of Education, Youth and Sports of the CR, Molecular pathophysiology of multigene diseases

Citovat

IZAKOVIČOVÁ HOLLÁ, Lydie, Dana BUČKOVÁ, Viera KUHROVÁ, Andrea STEJSKALOVÁ, Hana FRANCOVÁ, Vladimír ZNOJIL and Jirí VÁCHA. Prevalence of endothelial nitric oxide synthase gene polymorphisms in patients with atopic asthma. Clin Exp Allergy. England: Blackwell Science, 2002, vol. 32, No 8, p. 1193-1198. ISSN 0954-7894.

@article{403459,
   author = {Izakovičová Hollá, Lydie and Bučková, Dana and Kuhrová, Viera and Stejskalová, Andrea and Francová, Hana and Znojil, Vladimír and Vácha, Jirí},
   article_location = {England},
   article_number = {8},
   keywords = {nitric oxide synthase; gene; polymorphism; asthma; allergic disease},
   language = {eng},
   issn = {0954-7894},
   journal = {Clin Exp Allergy},
   title = {Prevalence of endothelial nitric oxide synthase gene polymorphisms in patients with atopic asthma},
   volume = {32},
   year = {2002}
}

TY  - JOUR
ID  - 403459
AU  - Izakovičová Hollá, Lydie - Bučková, Dana - Kuhrová, Viera - Stejskalová, Andrea - Francová, Hana - Znojil, Vladimír - Vácha, Jirí
PY  - 2002
TI  - Prevalence of endothelial nitric oxide synthase gene polymorphisms in patients with atopic asthma
JF  - Clin Exp Allergy
VL  - 32
IS  - 8
SP  - 1193
EP  - 1193
PB  - Blackwell Science
SN  - 09547894
KW  - nitric oxide synthase
KW  - gene
KW  - polymorphism
KW  - asthma
KW  - allergic disease
N2  - Asthma is a common multifactorial disease, the etiology of which is attributable to both environmental and genetic factors. The endothelial nitric oxide synthase (NOS3) gene has been implicated in asthma pathogenesis.This study investigated associations of 27 base-pair tandem repeat polymorphism in intron 4 and the Glu298Asp (G894T) variant of the NOS3 gene with atopic asthma in a Czech population. Polymerase chain reaction was used to determine the NOS3 genotypes in subjects with atopic asthma (n=163) and random controls (n=209). The NOS3 allele or genotype distributions did not significantly differ between the control and asthma groups. However, the common genotype (bb) of the NOS3 polymorphism in intron 4 was found to be significantly associated with total IgE levels (P=0.006), specific IgE levels for feathers (p=0.0002) and a positive skin prick test for hay (P=0.004). In one atopic patient, we identified an additional 27-bp repeat in the NOS3 gene (NOS3c), which occurred in heterozygous combination with the NOS3b allele (NOS3b/c genotype). In addition, we describe a new polymorphism (A5495G) in the NOS3 gene, which was in almost complete linkage disequilibrium with the NOS3 repeat polymorphism in intron 4. The Glu298Asp variant was not associated with asthma and/or related atopic phenotypes in our study. Neither the NOS3 b allele nor the NOS3 b/b genotype showed any general association with atopic asthma, but they were associated with atopy-related phenotypes. We conclude that the NOS3 gene polymorphisms may act as disease modifiers in atopic asthma phenotype in our population.
ER  -

IZAKOVIČOVÁ HOLLÁ, Lydie, Dana BUČKOVÁ, Viera KUHROVÁ, Andrea STEJSKALOVÁ, Hana FRANCOVÁ, Vladimír ZNOJIL and Jirí VÁCHA. Prevalence of endothelial nitric oxide synthase gene polymorphisms in patients with atopic asthma. \textit{Clin Exp Allergy}. England: Blackwell Science, 2002, vol.~32, No~8, p.~1193-1198. ISSN~0954-7894.

Detailed Information on Publication Record