J 2002

Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa

ŠVEC, Pavel, Luc DEVRIESE, Ivo SEDLÁČEK, Margo BAELE, Marc VANCANNEYT et. al.

Basic information

Original name

Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa

Authors

ŠVEC, Pavel (203 Czech Republic, guarantor), Luc DEVRIESE (56 Belgium), Ivo SEDLÁČEK (203 Czech Republic), Margo BAELE (56 Belgium), Marc VANCANNEYT (56 Belgium), Freddy HAESEBROUCK (56 Belgium), Jean SWINGS (56 Belgium) and Jiří DOŠKAŘ (203 Czech Republic)

Edition

Journal of Applied Microbiology, Oxford, UK, Blackwell Science Ltd, 2002, 1365-2672

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.819

RIV identification code

RIV/00216224:14310/02:00006086

Organization unit

Faculty of Science

UT WoS

000175184500020

Keywords in English

Bacterial Typing Techniques; DNA; Enterococcus; classification;Helix (Snails); Nucleic Acid Hybridization; Pigments/metabolism;16S Ribosomal RNA

Tags

International impact, Reviewed
Změněno: 18/6/2009 12:16, doc. RNDr. Pavel Švec, Ph.D.

Abstract

V originále

AIMS: Enterococci associated with garden snails (Helix aspersa) were studied in order to obtain reliable species identification and characterization. METHODS AND RESULTS: Twelve yellow-pigmented and motile enterococci, isolated from the intestines of garden snails, were phenotypically close to Enterococcus casseliflavus, but they showed certain unusual biochemical characteristics. tRNA intergenic length polymorphism analysis (tDNA-PCR) divided all strains studied into two groups, in full agreement with biochemical test results. 16S rDNA sequencing, DNA base composition analysis and DNA-DNA hybridization results showed unambiguously that the enterococci studied belonged to the species Ent. casseliflavus. The representative strains of described ecovars were deposited in the Czech Collection of Microorganisms (CCM) as Ent. casseliflavus CCM 4868, 4869, 4870 and 4871. CONCLUSIONS: Enterococcus casseliflavus associated with garden snails can be subdivided into groups. SIGNIFICANCE AND IMPACT OF THE STUDY: Enterococcus casseliflavus differs from other enterococcal species in that it is typically associated with plants, soil, water and invertebrate animals. The different groups that can be found in these widely occurring bacteria are possibly source-specific ecovars, as exemplified by the Ent. casseliflavus inhabiting the intestines of snails.

Links

GA301/02/1505, research and development project
Name: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků
Investor: Czech Science Foundation, Molecular diagnostic, epidemiology and classification of pathogenic Gram positive cocci