Characterization of yellow-pigmented and motile enterococci
isolated from intestines of the garden snail Helix aspersa

J 2002

Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa

ŠVEC, Pavel, Luc DEVRIESE, Ivo SEDLÁČEK, Margo BAELE, Marc VANCANNEYT et. al.

Basic information

Original name

Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa

Authors

ŠVEC, Pavel (203 Czech Republic, guarantor), Luc DEVRIESE (56 Belgium), Ivo SEDLÁČEK (203 Czech Republic), Margo BAELE (56 Belgium), Marc VANCANNEYT (56 Belgium), Freddy HAESEBROUCK (56 Belgium), Jean SWINGS (56 Belgium) and Jiří DOŠKAŘ (203 Czech Republic)

Edition

Journal of Applied Microbiology, Oxford, UK, Blackwell Science Ltd, 2002, 1365-2672

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

[Abstract] [Full text]

Impact factor

Impact factor: 1.819

RIV identification code

RIV/00216224:14310/02:00006086

Organization unit

Faculty of Science

UT WoS

000175184500020

Keywords in English

Bacterial Typing Techniques; DNA; Enterococcus; classification;Helix (Snails); Nucleic Acid Hybridization; Pigments/metabolism;16S Ribosomal RNA

Abstract

V originále

AIMS: Enterococci associated with garden snails (Helix aspersa) were studied in order to obtain reliable species identification and characterization. METHODS AND RESULTS: Twelve yellow-pigmented and motile enterococci, isolated from the intestines of garden snails, were phenotypically close to Enterococcus casseliflavus, but they showed certain unusual biochemical characteristics. tRNA intergenic length polymorphism analysis (tDNA-PCR) divided all strains studied into two groups, in full agreement with biochemical test results. 16S rDNA sequencing, DNA base composition analysis and DNA-DNA hybridization results showed unambiguously that the enterococci studied belonged to the species Ent. casseliflavus. The representative strains of described ecovars were deposited in the Czech Collection of Microorganisms (CCM) as Ent. casseliflavus CCM 4868, 4869, 4870 and 4871. CONCLUSIONS: Enterococcus casseliflavus associated with garden snails can be subdivided into groups. SIGNIFICANCE AND IMPACT OF THE STUDY: Enterococcus casseliflavus differs from other enterococcal species in that it is typically associated with plants, soil, water and invertebrate animals. The different groups that can be found in these widely occurring bacteria are possibly source-specific ecovars, as exemplified by the Ent. casseliflavus inhabiting the intestines of snails.

Links

GA301/02/1505, research and development project

Name: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků

Investor: Czech Science Foundation, Molecular diagnostic, epidemiology and classification of pathogenic Gram positive cocci

Citovat

ŠVEC, Pavel, Luc DEVRIESE, Ivo SEDLÁČEK, Margo BAELE, Marc VANCANNEYT, Freddy HAESEBROUCK, Jean SWINGS and Jiří DOŠKAŘ. Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa (Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa). Journal of Applied Microbiology. Oxford, UK: Blackwell Science Ltd, 2002, vol. 92, No 5, p. 951-957. ISSN 1365-2672.

@article{403808,
   author = {Švec, Pavel and Devriese, Luc and Sedláček, Ivo and Baele, Margo and Vancanneyt, Marc and Haesebrouck, Freddy and Swings, Jean and Doškař, Jiří},
   article_location = {Oxford, UK},
   article_number = {5},
   keywords = {Bacterial Typing Techniques; DNA; Enterococcus; classification;Helix (Snails); Nucleic Acid Hybridization; Pigments/metabolism;16S Ribosomal RNA},
   language = {eng},
   issn = {1365-2672},
   journal = {Journal of Applied Microbiology},
   title = {Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa},
   url = {http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2672.2002.01605.x},
   volume = {92},
   year = {2002}
}

TY  - JOUR
ID  - 403808
AU  - Švec, Pavel - Devriese, Luc - Sedláček, Ivo - Baele, Margo - Vancanneyt, Marc - Haesebrouck, Freddy - Swings, Jean - Doškař, Jiří
PY  - 2002
TI  - Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa
JF  - Journal of Applied Microbiology
VL  - 92
IS  - 5
SP  - 951
EP  - 951
PB  - Blackwell Science Ltd
SN  - 13652672
KW  - Bacterial Typing Techniques
KW  - DNA
KW  - Enterococcus
KW  - classification;Helix (Snails)
KW  - Nucleic Acid Hybridization
KW  - Pigments/metabolism;16S Ribosomal RNA
UR  - http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2672.2002.01605.x
N2  - AIMS: Enterococci associated with garden snails (Helix aspersa) were studied in order to obtain reliable species identification and characterization. METHODS AND RESULTS: Twelve yellow-pigmented and motile enterococci, isolated from the intestines of garden snails, were phenotypically close to Enterococcus casseliflavus, but they showed certain unusual biochemical characteristics. tRNA intergenic length polymorphism analysis (tDNA-PCR) divided all strains studied into two groups, in full agreement with biochemical test results. 16S rDNA sequencing, DNA base composition analysis and DNA-DNA hybridization results showed unambiguously that the enterococci studied belonged to the species Ent. casseliflavus. The representative strains of described ecovars were deposited in the Czech Collection of Microorganisms (CCM) as Ent. casseliflavus CCM 4868, 4869, 4870 and 4871. CONCLUSIONS: Enterococcus casseliflavus associated with garden snails can be subdivided into groups. SIGNIFICANCE AND IMPACT OF THE STUDY: Enterococcus casseliflavus differs from other enterococcal species in that it is typically associated with plants, soil, water and invertebrate animals. The different groups that can be found in these widely occurring bacteria are possibly source-specific ecovars, as exemplified by the Ent. casseliflavus inhabiting the intestines of snails.
ER  -

ŠVEC, Pavel, Luc DEVRIESE, Ivo SEDLÁČEK, Margo BAELE, Marc VANCANNEYT, Freddy HAESEBROUCK, Jean SWINGS and Jiří DOŠKAŘ. Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail $<$I$>$Helix aspersa$<$/I$>$ (Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa). \textit{Journal of Applied Microbiology}. Oxford, UK: Blackwell Science Ltd, 2002, vol.~92, No~5, p.~951-957. ISSN~1365-2672.

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