J 2002

Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping

TRNKOVÁ, Libuše, René KIZEK and Jan VACEK

Basic information

Original name

Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping

Authors

TRNKOVÁ, Libuše (203 Czech Republic, guarantor), René KIZEK (203 Czech Republic) and Jan VACEK (203 Czech Republic)

Edition

Bioelectrochemistry, The Netherlands, Elsevier, 2002, 1567-5394

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.463

RIV identification code

RIV/00216224:14310/02:00005813

Organization unit

Faculty of Science

Keywords in English

Metallothionein;Chronopotentiometric stripping analysis;Adsorptive transfer stripping;Peak H;Catalytic hydrogen evolution;Effect of aeration

Tags

Změněno: 24/5/2002 13:49, prof. RNDr. Libuše Trnková, CSc.

Abstract

V originále

Constant current chronopotentiometric stripping analysis (CPSA) in combination with adsorptive transfer stripping (AdTS) technique was used to study the rabbit liver metallothionein (MT) on a hanging mercury drop electrode (HMDE). Metallothionein yielded a distinct, sharp chronopotentiometric signal at very negative potentials (about -1.7 V ), also known as the "peak H". The height and potential of this peak were dependent on experimental conditions, such as buffer composition, pH, and the presence oxygen in solutions. The peak H was highest in borate buffer with pH close to the isoelectric point (pI) of MT. The chronopotentiometric results contribute to a deeper understanding of the nature of catalytic hydrogen evolution and demonstrate the usefulness of the peak H in peptide and protein research.

Links

GV204/97/K084, research and development project
Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu
Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research
MSM 143100011, plan (intention)
Name: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů
Investor: Ministry of Education, Youth and Sports of the CR, Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles