Promoter Polymorphisms in the CD14 Receptor Gene and their
Potential Association with the Severity of Chronic
Periodontitis

J 2002

Promoter Polymorphisms in the CD14 Receptor Gene and their Potential Association with the Severity of Chronic Periodontitis

IZAKOVIČOVÁ HOLLÁ, Lydie, Dana BUČKOVÁ, Antonín FASSMANN, Tomás HALABALA, Anna VASKŮ et. al.

Basic information

Original name

Promoter Polymorphisms in the CD14 Receptor Gene and their Potential Association with the Severity of Chronic Periodontitis

Authors

IZAKOVIČOVÁ HOLLÁ, Lydie (203 Czech Republic, guarantor), Dana BUČKOVÁ (203 Czech Republic), Antonín FASSMANN (203 Czech Republic), Tomás HALABALA (203 Czech Republic), Anna VASKŮ (203 Czech Republic) and Jirí VÁCHA (203 Czech Republic)

Edition

J Med Genet, London, BMJ Publishing Group, 2002, 0022-2593

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 7.774

RIV identification code

RIV/00216224:14110/02:00006167

Organization unit

Faculty of Medicine

UT WoS

000179208400012

Keywords in English

periodontitis; LPS; gene; polymorphism; CD14 receptor

Abstract

V originále

Chronic periodontitis, which is the major cause of tooth loss in adults, is characterized by a chronic inflammation of periodontal tissues mainly caused by infection with subgingival gram-negative, anaerobic bacteria. Cellular responses in periodontitis are mediated, in part, by bacterial lipopolysaccharide (LPS), which activates monocytes to express of cytokines, growth factors, and procoagulatory factors via LPS receptor CD14. The aim of this study was to determine whether the functional polymorphisms in the promoter of the CD14 gene are associated with the risk of chronic periodontitis and the severity of the disease. Two polymorphisms located in the promoter region (C(-159)T and G(-1359)T) were analysed by PCR and RFLP methods in a group of 135 patients with chronic periodontitis, and in 207 age- and sex- unrelated randomly selected control subjects. The CD14 allele and genotype distributions were similar between patients and controls. However, the frequency of the G allele of the G(-1359)T polymorphism was higher in patients with severe disease than moderate disease. The homozygous genotype (GG) of this polymorphism was found to be significantly increased in the severe patient group. Allele and/or genotype frequencies of the C(-159)T polymorphism did not differ significantly between severe and moderate periodontitis patients. However, we detected a tendency for an increased frequency of the -159 TT homozygotes in severe patients (19.2%) compared with the moderate subgroup (8.3%). In one patient, we identified a new mutation (28-bp deletion) in the promoter of the CD14 gene (del from -1310 to -1337), which occurred in a heterozygous combination. This study demonstrates that a specific genetic marker, which was previously associated with an increased sCD14 level, may act as a disease modifier of chronic periodontitis in the investigated population.

Links

MSM 141100002, plan (intention)

Name: Molekulární patofyziologie multigenních chorob

Investor: Ministry of Education, Youth and Sports of the CR, Molecular pathophysiology of multigene diseases

Citovat

IZAKOVIČOVÁ HOLLÁ, Lydie, Dana BUČKOVÁ, Antonín FASSMANN, Tomás HALABALA, Anna VASKŮ and Jirí VÁCHA. Promoter Polymorphisms in the CD14 Receptor Gene and their Potential Association with the Severity of Chronic Periodontitis (Promoter Polymorphisms in the CD14 Receptor Gene and their Potential Association with the Severity of Chronic PeriodontitisJ). J Med Genet. London: BMJ Publishing Group, 2002, vol. 39, No 11, p. 844-848. ISSN 0022-2593.

@article{404259,
   author = {Izakovičová Hollá, Lydie and Bučková, Dana and Fassmann, Antonín and Halabala, Tomás and Vasků, Anna and Vácha, Jirí},
   article_location = {London},
   article_number = {11},
   keywords = {periodontitis; LPS; gene; polymorphism; CD14 receptor},
   language = {eng},
   issn = {0022-2593},
   journal = {J Med Genet},
   title = {Promoter Polymorphisms in the CD14 Receptor Gene and their Potential Association with the Severity of Chronic Periodontitis},
   volume = {39},
   year = {2002}
}

TY  - JOUR
ID  - 404259
AU  - Izakovičová Hollá, Lydie - Bučková, Dana - Fassmann, Antonín - Halabala, Tomás - Vasků, Anna - Vácha, Jirí
PY  - 2002
TI  - Promoter Polymorphisms in the CD14 Receptor Gene and their Potential Association with the Severity of Chronic Periodontitis
JF  - J Med Genet
VL  - 39
IS  - 11
SP  - 844
EP  - 844
PB  - BMJ Publishing Group
SN  - 00222593
KW  - periodontitis
KW  - LPS
KW  - gene
KW  - polymorphism
KW  - CD14 receptor
N2  - Chronic periodontitis, which is the major cause of tooth loss in adults, is characterized by a chronic inflammation of periodontal tissues mainly caused by infection with subgingival gram-negative, anaerobic bacteria. Cellular responses in periodontitis are mediated, in part, by bacterial lipopolysaccharide (LPS), which activates monocytes to express of cytokines, growth factors, and procoagulatory factors via LPS receptor CD14. The aim of this study was to determine whether the functional polymorphisms in the promoter of the CD14 gene are associated with the risk of chronic periodontitis and the severity of the disease. Two polymorphisms located in the promoter region (C(-159)T and G(-1359)T) were analysed by PCR and RFLP methods in a group of 135 patients with chronic periodontitis, and in 207 age- and sex- unrelated randomly selected control subjects. The CD14 allele and genotype distributions were similar between patients and controls. However, the frequency of the G allele of the G(-1359)T polymorphism was higher in patients with severe disease than moderate disease. The homozygous genotype (GG) of this polymorphism was found to be significantly increased in the severe patient group. Allele and/or genotype frequencies of the C(-159)T polymorphism did not differ significantly between severe and moderate periodontitis patients. However, we detected a tendency for an increased frequency of the -159 TT homozygotes in severe patients (19.2%) compared with the moderate subgroup (8.3%). In one patient, we identified a new mutation (28-bp deletion) in the promoter of the CD14 gene (del from -1310 to -1337), which occurred in a heterozygous combination. This study demonstrates that a specific genetic marker, which was previously associated with an increased sCD14 level, may act as a disease modifier of chronic periodontitis in the investigated population.
ER  -

IZAKOVIČOVÁ HOLLÁ, Lydie, Dana BUČKOVÁ, Antonín FASSMANN, Tomás HALABALA, Anna VASKŮ and Jirí VÁCHA. Promoter Polymorphisms in the CD14 Receptor Gene and their Potential Association with the Severity of Chronic Periodontitis (Promoter Polymorphisms in the CD14 Receptor Gene and their Potential Association with the Severity of Chronic PeriodontitisJ). \textit{J Med Genet}. London: BMJ Publishing Group, 2002, vol.~39, No~11, p.~844-848. ISSN~0022-2593.

Detailed Information on Publication Record