Automated micro axial tomography of cell nuclei after specific
labelling by fluorescence in situ hybridisation

J 2002

Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation

KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Joachim RAUCH et. al.

Basic information

Original name

Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation

Authors

KOZUBEK, Michal (203 Czech Republic, guarantor), Magdalena SKALNÍKOVÁ (203 Czech Republic), Petr MATULA (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Joachim RAUCH (276 Germany), Friedrich NEUHAUS (276 Germany), Heinz EIPEL (276 Germany) and Michael HAUSMANN (276 Germany)

Edition

Micron, Amsterdam, Elsevier, 2002, 0968-4328

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.537

RIV identification code

RIV/00216224:14330/02:00006215

Organization unit

Faculty of Informatics

Keywords in English

micro axial tomography; automated microscopy; high-resolution cytometry; FISH imaging; interphase cell nuclei

Abstract

V originále

Micro axial tomography provides a good means for microscopic image acquisition of cells or sub-cellular components like cell nuclei with an improved resolution, because shortcomings of spatial resolution anisotropy in optical microscopy can be overcome. Thus, spatial information of the object can be obtained without the necessity of confocal imaging. Since the very early developments of micro axial tomography a considerable drawback of this method was a complicated image acquisition and processing procedure that requires much operator time. In order to solve this problem the Heidelberg 2pi-tilting device has been mounted to the Brno high-resolution cytometer as an attempt to bring together advanced microscopy and fast automated computer image acquisition and analysis. A special software module that drives all hardware components required for automated micro axial tomography and performs image acquisition and processing has been developed. Also a method for the preparation of cell nuclei attached to glass fibres has been developed that allows for the first time imaging of three-dimensionally conserved, FISH-stained cell nuclei fixed to a glass fibre.

Links

MSM 143300002, plan (intention)

Name: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy

Citovat

KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Joachim RAUCH, Friedrich NEUHAUS, Heinz EIPEL and Michael HAUSMANN. Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation. Micron. Amsterdam: Elsevier, 2002, vol. 33, No 7, p. 655-665. ISSN 0968-4328.

@article{404450,
   author = {Kozubek, Michal and Skalníková, Magdalena and Matula, Petr and Bártová, Eva and Rauch, Joachim and Neuhaus, Friedrich and Eipel, Heinz and Hausmann, Michael},
   article_location = {Amsterdam},
   article_number = {7},
   keywords = {micro axial tomography; automated microscopy; high-resolution cytometry; FISH imaging; interphase cell nuclei},
   language = {eng},
   issn = {0968-4328},
   journal = {Micron},
   title = {Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation},
   volume = {33},
   year = {2002}
}

TY  - JOUR
ID  - 404450
AU  - Kozubek, Michal - Skalníková, Magdalena - Matula, Petr - Bártová, Eva - Rauch, Joachim - Neuhaus, Friedrich - Eipel, Heinz - Hausmann, Michael
PY  - 2002
TI  - Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation
JF  - Micron
VL  - 33
IS  - 7
SP  - 655
EP  - 655
PB  - Elsevier
SN  - 09684328
KW  - micro axial tomography
KW  - automated microscopy
KW  - high-resolution cytometry
KW  - FISH imaging
KW  - interphase cell nuclei
N2  - Micro axial tomography provides a good means for microscopic image acquisition of cells or sub-cellular components like cell nuclei with an improved resolution, because shortcomings of spatial resolution anisotropy in optical microscopy can be overcome. Thus, spatial information of the object can be obtained without the necessity of confocal imaging. Since the very early developments of micro axial tomography a considerable drawback of this method was a complicated image acquisition and processing procedure that requires much operator time. In order to solve this problem the Heidelberg 2pi-tilting device has been mounted to the Brno high-resolution cytometer as an attempt to bring together advanced microscopy and fast automated computer image acquisition and analysis. A special software module that drives all hardware components required for automated micro axial tomography and performs image acquisition and processing has been developed. Also a method for the preparation of cell nuclei attached to glass fibres has been developed that allows for the first time imaging of three-dimensionally conserved, FISH-stained cell nuclei fixed to a glass fibre.
ER  -

KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Joachim RAUCH, Friedrich NEUHAUS, Heinz EIPEL and Michael HAUSMANN. Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation. \textit{Micron}. Amsterdam: Elsevier, 2002, vol.~33, No~7, p.~655-665. ISSN~0968-4328.

Detailed Information on Publication Record