KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Joachim RAUCH, Friedrich NEUHAUS, Heinz EIPEL and Michael HAUSMANN. Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation. Micron. Amsterdam: Elsevier, vol. 33, No 7, p. 655-665. ISSN 0968-4328. 2002.
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Basic information
Original name Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation
Authors KOZUBEK, Michal (203 Czech Republic, guarantor), Magdalena SKALNÍKOVÁ (203 Czech Republic), Petr MATULA (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Joachim RAUCH (276 Germany), Friedrich NEUHAUS (276 Germany), Heinz EIPEL (276 Germany) and Michael HAUSMANN (276 Germany).
Edition Micron, Amsterdam, Elsevier, 2002, 0968-4328.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 20200 2.2 Electrical engineering, Electronic engineering, Information engineering
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.537
RIV identification code RIV/00216224:14330/02:00006215
Organization unit Faculty of Informatics
Keywords in English micro axial tomography; automated microscopy; high-resolution cytometry; FISH imaging; interphase cell nuclei
Tags automated microscopy, cbia-web, FISH imaging, high-resolution cytometry, interphase cell nuclei, micro axial tomography
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Petr Matula, Ph.D., učo 3019. Changed: 6/5/2009 10:59.
Abstract
Micro axial tomography provides a good means for microscopic image acquisition of cells or sub-cellular components like cell nuclei with an improved resolution, because shortcomings of spatial resolution anisotropy in optical microscopy can be overcome. Thus, spatial information of the object can be obtained without the necessity of confocal imaging. Since the very early developments of micro axial tomography a considerable drawback of this method was a complicated image acquisition and processing procedure that requires much operator time. In order to solve this problem the Heidelberg 2pi-tilting device has been mounted to the Brno high-resolution cytometer as an attempt to bring together advanced microscopy and fast automated computer image acquisition and analysis. A special software module that drives all hardware components required for automated micro axial tomography and performs image acquisition and processing has been developed. Also a method for the preparation of cell nuclei attached to glass fibres has been developed that allows for the first time imaging of three-dimensionally conserved, FISH-stained cell nuclei fixed to a glass fibre.
Links
MSM 143300002, plan (intention)Name: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
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