KOZUBEK, Michal, Petr MATULA, Heinz EIPEL and Michael HAUSMANN. Automated multi-view 3D image acquisition in human genome research. In 3D data processing, visualization and transmission. Los Alamitos: IEEE Computer Society, 2002, p. 91-98. ISBN 0-7695-1521-5.
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Basic information
Original name Automated multi-view 3D image acquisition in human genome research
Authors KOZUBEK, Michal (203 Czech Republic, guarantor, belonging to the institution), Petr MATULA (203 Czech Republic, belonging to the institution), Heinz EIPEL (276 Germany) and Michael HAUSMANN (276 Germany).
Edition Los Alamitos, 3D data processing, visualization and transmission, p. 91-98, 2002.
Publisher IEEE Computer Society
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 20200 2.2 Electrical engineering, Electronic engineering, Information engineering
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Publication form printed version "print"
RIV identification code RIV/00216224:14330/02:00006216
Organization unit Faculty of Informatics
ISBN 0-7695-1521-5
UT WoS 000177028700016
Keywords in English micro axial tomography; automated microscopy; high-resolution cytometry; FISH imaging; interphase cell nuclei
Tags automated microscopy, FISH imaging, high-resolution cytometry, interphase cell nuclei, micro axial tomography
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Petr Matula, Ph.D., učo 3019. Changed: 12/2/2018 15:03.
Abstract
One of the main problems of 3D imaging in optical microscopy is unisotropic optical resolution of acquired 3D data. For instance in confocal laser scanning microscopy, axial resolution is 2-3 times worse than the lateral one. A sophisticated solution of this problem is so-called micro axial tomography that provides a good means for microscopic image acquisition of cells or sub-cellular components like cell nuclei with an improved resolution achieved by object tilting and acquisition of a series of mutually tilted 3D image data. Since the very early developments of micro axial tomography a considerable drawback of this method was a complicated image acquisition and processing procedure that requires much operator time. In order to solve this problem advanced microscopy and automated computer image acquisition and analysis were brought together. Special software that drives all hardware components required for automated micro axial tomography was developed. It performs multi-view 3D image acquisition as well as related image processing.
Links
MSM 143300002, plan (intention)Name: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
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