Development of software for automated micro-axialtomography

D 2002

Development of software for automated micro-axialtomography

KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Heinz EIPEL et. al.

Basic information

Original name

Development of software for automated micro-axialtomography

Authors

KOZUBEK, Michal (203 Czech Republic, guarantor), Magdalena SKALNÍKOVÁ (203 Czech Republic), Petr MATULA (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Heinz EIPEL (276 Germany) and Michael HAUSMANN (276 Germany)

Edition

Brno, Biophysics of the genome and its interactions, p. 45-53, 2002

Publisher

Masaryk University

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14330/02:00006218

Organization unit

Faculty of Informatics

ISBN

80-210-2853-X

Keywords in English

micro axial tomography; automated microscopy; high-resolution cytometry; FISH imaging; interphase cell nuclei

Abstract

V originále

Micro-axialtomography allows the observation of cells, sub-cellular organelles and their compounds (such as genetic loci or proteins) from an arbitrary angle of view. This is accomplished by means of fixation of the objects to a thin glass fibre and rotation of the fibre around its axis perpendicularly to the optical axis. The cells and their compounds are typically stained by immuno-fluorescence techniques as for instance FISH to label chromatin targets in intact cell nuclei. The rotated objects can be observed in a fluorescence microscope. In order to facilitate the acquisition and analysis of a large number of objects, the development of an appropriate software module has begun that is incorporated into the image acquisition and analysis package FISH 2.0. Currently the module contains trial versions of the algorithms for image acquisition and analysis and the whole system is being tested. Examples of images obtained using micro-axialtomography are presented as well as the description of the algorithms for image acquisition and analysis.

Links

MSM 143300002, plan (intention)

Name: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy

Citovat

KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Heinz EIPEL and Michael HAUSMANN. Development of software for automated micro-axialtomography. In Biophysics of the genome and its interactions. Brno: Masaryk University, 2002, p. 45-53. ISBN 80-210-2853-X.

@inproceedings{404454,
   author = {Kozubek, Michal and Skalníková, Magdalena and Matula, Petr and Bártová, Eva and Eipel, Heinz and Hausmann, Michael},
   address = {Brno},
   booktitle = {Biophysics of the genome and its interactions},
   keywords = {micro axial tomography; automated microscopy; high-resolution cytometry; FISH imaging; interphase cell nuclei},
   language = {eng},
   location = {Brno},
   isbn = {80-210-2853-X},
   pages = {45-53},
   publisher = {Masaryk University},
   title = {Development of software for automated micro-axialtomography},
   year = {2002}
}

TY  - JOUR
ID  - 404454
AU  - Kozubek, Michal - Skalníková, Magdalena - Matula, Petr - Bártová, Eva - Eipel, Heinz - Hausmann, Michael
PY  - 2002
TI  - Development of software for automated micro-axialtomography
PB  - Masaryk University
CY  - Brno
SN  - 802102853X
KW  - micro axial tomography
KW  - automated microscopy
KW  - high-resolution cytometry
KW  - FISH imaging
KW  - interphase cell nuclei
N2  - Micro-axialtomography allows the observation of cells, sub-cellular organelles and their compounds (such as genetic loci or proteins) from an arbitrary angle of view. This is accomplished by means of fixation of the objects to a thin glass fibre and rotation of the fibre around its axis perpendicularly to the optical axis. The cells and their compounds are typically stained by immuno-fluorescence techniques as for instance FISH to label chromatin targets in intact cell nuclei. The rotated objects can be observed in a fluorescence microscope. In order to facilitate the acquisition and analysis of a large number of objects, the development of an appropriate software module has begun that is incorporated into the image acquisition and analysis package FISH 2.0. Currently the module contains trial versions of the algorithms for image acquisition and analysis and the whole system is being tested. Examples of images obtained using micro-axialtomography are presented as well as the description of the algorithms for image acquisition and analysis.
ER  -

KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Heinz EIPEL and Michael HAUSMANN. Development of software for automated micro-axialtomography. In \textit{Biophysics of the genome and its interactions}. Brno: Masaryk University, 2002, p.~45-53. ISBN~80-210-2853-X.

Detailed Information on Publication Record