KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Heinz EIPEL and Michael HAUSMANN. Development of software for automated micro-axialtomography. In Biophysics of the genome and its interactions. Brno: Masaryk University, 2002, p. 45-53. ISBN 80-210-2853-X.
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Basic information
Original name Development of software for automated micro-axialtomography
Authors KOZUBEK, Michal (203 Czech Republic, guarantor), Magdalena SKALNÍKOVÁ (203 Czech Republic), Petr MATULA (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Heinz EIPEL (276 Germany) and Michael HAUSMANN (276 Germany).
Edition Brno, Biophysics of the genome and its interactions, p. 45-53, 2002.
Publisher Masaryk University
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 20200 2.2 Electrical engineering, Electronic engineering, Information engineering
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14330/02:00006218
Organization unit Faculty of Informatics
ISBN 80-210-2853-X
Keywords in English micro axial tomography; automated microscopy; high-resolution cytometry; FISH imaging; interphase cell nuclei
Tags automated microscopy, FISH imaging, high-resolution cytometry, interphase cell nuclei, micro axial tomography
Changed by Changed by: prof. RNDr. Michal Kozubek, Ph.D., učo 3740. Changed: 10/7/2002 11:20.
Abstract
Micro-axialtomography allows the observation of cells, sub-cellular organelles and their compounds (such as genetic loci or proteins) from an arbitrary angle of view. This is accomplished by means of fixation of the objects to a thin glass fibre and rotation of the fibre around its axis perpendicularly to the optical axis. The cells and their compounds are typically stained by immuno-fluorescence techniques as for instance FISH to label chromatin targets in intact cell nuclei. The rotated objects can be observed in a fluorescence microscope. In order to facilitate the acquisition and analysis of a large number of objects, the development of an appropriate software module has begun that is incorporated into the image acquisition and analysis package FISH 2.0. Currently the module contains trial versions of the algorithms for image acquisition and analysis and the whole system is being tested. Examples of images obtained using micro-axialtomography are presented as well as the description of the algorithms for image acquisition and analysis.
Links
MSM 143300002, plan (intention)Name: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
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