Role of SdiA in Salmonella enterica serovar typhimurium physiology and virulence

VOLF, Jiří, Mojmír ŠEVČÍK, Hana HAVLÍČKOVÁ, František ŠIŠÁK, Jiří DAMBORSKÝ and Ivan RYCHLÍK. Role of SdiA in Salmonella enterica serovar typhimurium physiology and virulence. Archives in Microbiology. 2002, vol. 178, No 2, p. 94-101. ISSN 0302-8933.

Basic information

• Original name: Role of SdiA in Salmonella enterica serovar typhimurium physiology and virulence
• Authors: VOLF, Jiří (203 Czech Republic), Mojmír ŠEVČÍK (203 Czech Republic), Hana HAVLÍČKOVÁ (203 Czech Republic), František ŠIŠÁK (203 Czech Republic), Jiří DAMBORSKÝ (203 Czech Republic) and Ivan RYCHLÍK (203 Czech Republic, guarantor).
• Edition: Archives in Microbiology, 2002, 0302-8933.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 1.903
• RIV identification code: RIV/00216224:14310/02:00006266
• Organization unit: Faculty of Science
• UT WoS: 000177238900003
• Keywords in English: VIRULENCE; TRANSCRIPTION; SALMONELLA; SDI GENE; MUTANT
• Tags: MUTANT, Salmonella, SDI GENE, Transcription, VIRULENCE

Abstract

sdiA in Salmonella enterica serovar Typhimurium encodes a protein belonging to the LuxR family of transcriptional regulators. Initial computer analysis revealed the presence of a fur box 19 bp upstream of the start codon of the sdiA gene and a helix-turn-helix motif in the carboxy-terminal part of the SdiA protein typical for transcriptional regulators. Deletion of the fur box resulted in twofold increase of sdiA transcription. Furthermore, addition of dipyridyl, an iron chelator, to culture media increased sdiA transcription to the level observed in the fur box mutant, confirming that sdiA is suppressed in the presence of iron. When S. enterica sv. Typhimurium was grown in conditioned medium, sdiA transcription was repressed to 30% of its transcription in cells grown in fresh LB broth and this repression was independent of the fur box. Oral infection of mice with the strain lacking the helix-turn-helix domain of SdiA indicated increased virulence of such S. enterica sv. Typhimurium mutant. sdiA, dually controlled by iron concentration and culture density derived signals, may therefore play an important role in S. enterica sv. Typhimurium virulence regulation.

Links

• LN00A016, research and development project: Name: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Center