J 2002

Role of SdiA in Salmonella enterica serovar typhimurium physiology and virulence

VOLF, Jiří, Mojmír ŠEVČÍK, Hana HAVLÍČKOVÁ, František ŠIŠÁK, Jiří DAMBORSKÝ et. al.

Basic information

Original name

Role of SdiA in Salmonella enterica serovar typhimurium physiology and virulence

Authors

VOLF, Jiří (203 Czech Republic), Mojmír ŠEVČÍK (203 Czech Republic), Hana HAVLÍČKOVÁ (203 Czech Republic), František ŠIŠÁK (203 Czech Republic), Jiří DAMBORSKÝ (203 Czech Republic) and Ivan RYCHLÍK (203 Czech Republic, guarantor)

Edition

Archives in Microbiology, 2002, 0302-8933

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.903

RIV identification code

RIV/00216224:14310/02:00006266

Organization unit

Faculty of Science

UT WoS

000177238900003

Keywords in English

VIRULENCE; TRANSCRIPTION; SALMONELLA; SDI GENE; MUTANT

Tags

Změněno: 19/3/2010 10:54, prof. Mgr. Jiří Damborský, Dr.

Abstract

V originále

sdiA in Salmonella enterica serovar Typhimurium encodes a protein belonging to the LuxR family of transcriptional regulators. Initial computer analysis revealed the presence of a fur box 19 bp upstream of the start codon of the sdiA gene and a helix-turn-helix motif in the carboxy-terminal part of the SdiA protein typical for transcriptional regulators. Deletion of the fur box resulted in twofold increase of sdiA transcription. Furthermore, addition of dipyridyl, an iron chelator, to culture media increased sdiA transcription to the level observed in the fur box mutant, confirming that sdiA is suppressed in the presence of iron. When S. enterica sv. Typhimurium was grown in conditioned medium, sdiA transcription was repressed to 30% of its transcription in cells grown in fresh LB broth and this repression was independent of the fur box. Oral infection of mice with the strain lacking the helix-turn-helix domain of SdiA indicated increased virulence of such S. enterica sv. Typhimurium mutant. sdiA, dually controlled by iron concentration and culture density derived signals, may therefore play an important role in S. enterica sv. Typhimurium virulence regulation.

Links

LN00A016, research and development project
Name: BIOMOLEKULÁRNÍ CENTRUM
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Center