Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography

SKALNÍKOVÁ, Magdalena, Michal KOZUBEK, Petr MATULA, Eva BÁRTOVÁ, Heinz EIPEL and Michael HAUSMANN. Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography. In Biophysics of the Genome and Its Interactions. Brno: Masaryk University, 2002, p. 82-85. ISBN 80-210-2853-X.

Basic information

Original name

Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography

Authors

SKALNÍKOVÁ, Magdalena (203 Czech Republic), Michal KOZUBEK (203 Czech Republic, guarantor), Petr MATULA (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Heinz EIPEL (276 Germany) and Michael HAUSMANN (276 Germany)

Edition

Brno, Biophysics of the Genome and Its Interactions, p. 82-85, 2002

Publisher

Masaryk University

---

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10610 Biophysics

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14330/02:00006269

Organization unit

Faculty of Informatics

ISBN

80-210-2853-X

Keywords in English

Chromatin structure; FISH; Micro axial tomography; High-resolution cytometry; fibre glass

Tags

chromatin structure, fibre glass, FISH, high-resolution cytometry, micro axial tomography

---

Abstract

V originále

Micro axial tomography is a new method for microscopic image acquisition of fluorescence in situ hybridisation (FISH) stained cell nuclei with improved spatial resolution. Automated axial tomography has grown up from a common Brno-Heidelberg collaboration of two scientific groups, which are interested in the study of higher order chromatin structure in FISH stained cells using epifluorescence and confocal laser scanning fluorescence microscopy. The aim of the common Brno-Heidelberg project is to combine high-resolution cytometry (HRCM) and 2đ-tilting device in order to obtain statistically significant data about the structure of cell nuclei using advanced microscopy and fast automated computer image acquisition and analysis. In this study a method for the preparation of cell nuclei attached to glass fibres has been developed. The optimised experimental procedure for the first time provides imaging of three-dimensionally conserved, FISH-stained cell nuclei fixed to a glass fibre

---

Links

GA301/01/0186, research and development project	Name: Studium lokální kontroly exprese genů pomocí spektrální mikroskopie a analýzy obrazu Investor: Czech Science Foundation, Investigation of the local control of gene expression using spectral microscopy and omage analysis techniques
IBS5004010, research and development project	Name: Vývoj nových diagnostických technik pro onkologii Investor: Academy of Sciences of the Czech Republic, Development of new diagnostic teniques for oncology
MSM 143300002, plan (intention)	Name: Využití počítačové analýzy obrazu v optické mikroskopii Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
NC5955, research and development project	Name: Jak může přispět studium prostorového uspořádání specifických genetických lokusů v jádře buněk zdravých a maligních tkání k diagnostice a léčbě solidních tumorů. Investor: Ministry of Health of the CR, How the study spatial order of specific genetic loci in nuclei of healthy and malignant tissues can contribute to the diagnostics and treatment of solid tumors.