Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography

SKALNÍKOVÁ, Magdalena, Michal KOZUBEK, Petr MATULA, Eva BÁRTOVÁ, Heinz EIPEL and Michael HAUSMANN. Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography. In Biophysics of the Genome and Its Interactions. Brno: Masaryk University, 2002, p. 82-85. ISBN 80-210-2853-X.

Basic information

• Original name: Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography
• Authors: SKALNÍKOVÁ, Magdalena (203 Czech Republic), Michal KOZUBEK (203 Czech Republic, guarantor), Petr MATULA (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Heinz EIPEL (276 Germany) and Michael HAUSMANN (276 Germany).
• Edition: Brno, Biophysics of the Genome and Its Interactions, p. 82-85, 2002.
• Publisher: Masaryk University

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10610 Biophysics
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14330/02:00006269
• Organization unit: Faculty of Informatics
• ISBN: 80-210-2853-X
• Keywords in English: Chromatin structure; FISH; Micro axial tomography; High-resolution cytometry; fibre glass
• Tags: chromatin structure, fibre glass, FISH, high-resolution cytometry, micro axial tomography

Abstract

Micro axial tomography is a new method for microscopic image acquisition of fluorescence in situ hybridisation (FISH) stained cell nuclei with improved spatial resolution. Automated axial tomography has grown up from a common Brno-Heidelberg collaboration of two scientific groups, which are interested in the study of higher order chromatin structure in FISH stained cells using epifluorescence and confocal laser scanning fluorescence microscopy. The aim of the common Brno-Heidelberg project is to combine high-resolution cytometry (HRCM) and 2đ-tilting device in order to obtain statistically significant data about the structure of cell nuclei using advanced microscopy and fast automated computer image acquisition and analysis. In this study a method for the preparation of cell nuclei attached to glass fibres has been developed. The optimised experimental procedure for the first time provides imaging of three-dimensionally conserved, FISH-stained cell nuclei fixed to a glass fibre

Links

• GA301/01/0186, research and development project: Name: Studium lokální kontroly exprese genů pomocí spektrální mikroskopie a analýzy obrazu

Investor: Czech Science Foundation, Investigation of the local control of gene expression using spectral microscopy and omage analysis techniques

• IBS5004010, research and development project: Name: Vývoj nových diagnostických technik pro onkologii

Investor: Academy of Sciences of the Czech Republic, Development of new diagnostic teniques for oncology

• MSM 143300002, plan (intention): Name: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy

• NC5955, research and development project: Name: Jak může přispět studium prostorového uspořádání specifických genetických lokusů v jádře buněk zdravých a maligních tkání k diagnostice a léčbě solidních tumorů.

Investor: Ministry of Health of the CR, How the study spatial order of specific genetic loci in nuclei of healthy and malignant tissues can contribute to the diagnostics and treatment of solid tumors.